End-column amperometric detection of 6-mercaptopurine by capillary zone electrophoresis

被引：0

作者：

Wang, XP ^{[1
]}

You, TY ^{[1
]}

Yang, XR ^{[1
]}

Wang, EK ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Electroanalyt Chem Lab, Natl Analyt Res Ctr Electrochem & Spect, Changchun 130022, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 1999年 / 27卷 / 10期

关键词：

capillary electrophoresis; amperometric detection; 6-mercaptopurine;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

End-column amperometric detection of 6-mercaptopurine by capillary zone electrophoresis was described with high resolution and speed. The detection conditions were optimized and the electrochemical behavior was observed. Under the optimal conditions: detection potential of 1.2 V ( vs. Ag/AgCl), operating voltage of 15 kV, sample injection of 3 s at 15 kV and 10 mmol/l Na2HPO4 buffer, the detection limit for 6-MP was as low as 1 x 10(-7) mol/L and the linear range was from 5 x 10(-4) to 5 x 10(-6) mol/L with the relative coefficient of 0.995. The RSD of reproducibility for peak current and migration time was 2.5% and 1.2%, respectively. This method was utilized in assay real sample of human mine and bovine serum albumin containing 6-mercaptopurine.

引用

页码：1141 / 1145

页数：5

共 13 条

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