Soybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH4+ transport

被引:75
作者
Chiasson, David M. [1 ]
Loughlin, Patrick C. [1 ,2 ]
Mazurkiewicz, Danielle [1 ]
Mohammadidehcheshmeh, Manijeh [1 ]
Fedorova, Elena E. [3 ]
Okamoto, Mamoru [1 ]
McLean, Elizabeth [4 ]
Glass, Anthony D. M. [5 ]
Smith, Sally E. [1 ]
Bisseling, Ton [3 ,6 ]
Tyerman, Stephen D. [1 ]
Day, David A. [7 ]
Kaiser, Brent N. [1 ]
机构
[1] Univ Adelaide, Sch Agr Food & Wine, Adelaide, SA 5050, Australia
[2] Univ Sydney, Sch Biol Sci, Sydney, NSW 2006, Australia
[3] Wageningen Univ, Dept Plant Sci, Lab Mol Biol, NL-6703 HA Wageningen, Netherlands
[4] Univ Western Australia, Sch Plant Biol, Perth, WA 6009, Australia
[5] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada
[6] King Saud Univ, Coll Sci, Riyadh 11451, Saudi Arabia
[7] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia
基金
澳大利亚研究理事会;
关键词
nitrogen fixation; legume; nitrogen transport; CIRCADIAN CLOCK; STRESS-RESPONSE; ER STRESS; MEMBRANE; ARABIDOPSIS; PROTEIN; FAMILY; IDENTIFICATION; FIXATION; NITROGEN;
D O I
10.1073/pnas.1312801111
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Glycine max symbiotic ammonium transporter 1 was first documented as a putative ammonium (NH4+) channel localized to the symbiosome membrane of soybean root nodules. We show that Glycine max symbiotic ammonium transporter 1 is actually a membrane-localized basic helix-loop-helix (bHLH) DNA-binding transcription factor now renamed Glycine max bHLH membrane 1 (GmbHLHm1). In yeast, GmbHLHm1 enters the nucleus and transcriptionally activates a unique plasma membrane NH4+ channel Saccharomyces cerevisiae ammonium facilitator 1. Ammonium facilitator 1 homologs are present in soybean and other plant species, where they often share chromosomal microsynteny with bHLHm1 loci. GmbHLHm1 is important to the soybean rhizobium symbiosis because loss of activity results in a reduction of nodule fitness and growth. Transcriptional changes in nodules highlight downstream signaling pathways involving circadian clock regulation, nutrient transport, hormone signaling, and cell wall modification. Collectively, these results show that GmbHLHm1 influences nodule development and activity and is linked to a novel mechanism for NH4+ transport common to both yeast and plants.
引用
收藏
页码:4814 / 4819
页数:6
相关论文
共 43 条
  • [1] Positional dependence, cliques, and predictive motifs in the bHLH protein domain
    Atchley, WR
    Terhalle, W
    Dress, A
    [J]. JOURNAL OF MOLECULAR EVOLUTION, 1999, 48 (05) : 501 - 516
  • [2] Comparative physical mapping links conservation of microsynteny to chromosome structure and recombination in grasses
    Bowers, JE
    Arias, MA
    Asher, R
    Avise, JA
    Ball, RT
    Brewer, GA
    Buss, RW
    Chen, AH
    Edwards, TM
    Estill, JC
    Exum, HE
    Goff, VH
    Herrick, KL
    Steele, CLJ
    Karunakaran, S
    Lafayette, GK
    Lemke, C
    Marler, BS
    Masters, SL
    McMillan, JM
    Nelson, LK
    Newsome, GA
    Nwakanma, CC
    Odeh, RN
    Phelps, CA
    Rarick, EA
    Rogers, CJ
    Ryan, SP
    Slaughter, KA
    Soderlund, CA
    Tang, HB
    Wing, RA
    Paterson, AH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) : 13206 - 13211
  • [3] Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance
    Bozdag, Gonensin Ozan
    Uluisik, Irem
    Gulculer, Gulce Sila
    Karakaya, Huseyin C.
    Koc, Ahmet
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2011, 409 (04) : 748 - 751
  • [4] The circadian clock regulates auxin signaling and responses in Arabidopsis
    Covington, Michael F.
    Harmer, Stacey L.
    [J]. PLOS BIOLOGY, 2007, 5 (08) : 1773 - 1784
  • [5] Characterization of three functional high-affinity ammonium transporters in Lotus japonicus with differential transcriptional regulation spatial expression
    D'Apuzzo, E
    Rogato, A
    Simon-Rosin, U
    El Alaoui, H
    Barbulova, A
    Betti, M
    Dimou, M
    Katinakis, P
    Marquez, A
    Marini, AM
    Udvardi, MK
    Chiurazzi, M
    [J]. PLANT PHYSIOLOGY, 2004, 134 (04) : 1763 - 1774
  • [6] The circadian oscillator gene GIGANTEA mediates a long-term response of the Arabidopsis thaliana circadian clock to sucrose
    Dalchau, Neil
    Baek, Seong J.
    Briggs, Helen M.
    Robertson, Fiona C.
    Dodd, Antony N.
    Gardner, Michael J.
    Stancombe, Matthew A.
    Haydon, Michael J.
    Stan, Guy-Bart
    Goncalves, Jorge M.
    Webb, Alex A. R.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (12) : 5104 - 5109
  • [7] RECOGNITION BY MAX OF ITS COGNATE DNA THROUGH A DIMERIC B/HLH/Z DOMAIN
    FERREDAMARE, AR
    PRENDERGAST, GC
    ZIFF, EB
    BURLEY, SK
    [J]. NATURE, 1993, 363 (6424) : 38 - 45
  • [8] A membrane-tethered transcription factor defines a branch of the heat stress response in Arabidopsis thaliana
    Gao, Hongbo
    Brandizzi, Federica
    Benning, Christoph
    Larkin, Robert M.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (42) : 16398 - 16403
  • [9] Protein sensors for membrane sterols
    Goldstein, JL
    DeBose-Boyd, RA
    Brown, MS
    [J]. CELL, 2006, 124 (01) : 35 - 46
  • [10] The basic helix-loop-helix transcription factor family in plants: A genome-wide study of protein structure and functional diversity
    Heim, MA
    Jakoby, M
    Werber, M
    Martin, C
    Weisshaar, B
    Bailey, PC
    [J]. MOLECULAR BIOLOGY AND EVOLUTION, 2003, 20 (05) : 735 - 747