High performance liquid chromatography with evaporative light scattering detection for the characterisation of a vesicular delivery system during stability studies

被引：6
作者：
Alsaadi, Manal M. [1 ]
Carter, K. Christine [1 ]
Mullen, Alexander B. [1 ]
机构：
[1] Univ Strathclyde, Strathclyde Inst Pharm & Biomed Sci, Glasgow G4 0RE, Lanark, Scotland
来源：
JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1320卷
关键词：
Non-ionic surfactant; Vesicles; Lipid analysis; Normal phase HPLC; Evaporative light scattering detection; NONIONIC SURFACTANT VESICLES; SODIUM STIBOGLUCONATE; RESPONSE ENHANCEMENT; AMPHOTERICIN-B; LIPID CLASSES; NIOSOMES; LIPOSOMES; LEISHMANIASIS; FORMULATION; PHASE;
D O I：
10.1016/j.chroma.2013.10.054
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A normal phase high performance liquid chromatography method with evaporative light scattering detection was developed for the simultaneous quantification of the lipid constituents of a non-ionic surfactant vesicle (NIV) delivery system consisting of tetra-ethylene glycol mono n-hexadecyl ether, cholesterol and dicetyl phosphate. An accelerated stability study performed at 25 degrees C/60% relative humidity (RH) and 40 degrees C/75% RH indicated that the NIV were chemically stable. Similar results were observed when stored at 4 degrees C for 469 days. This chromatographic method developed is a sensitive, robust and high throughput analytical technique that offers the potential for rapid quantification of lipids in liposomal and vesicular systems. The results of the chromatographic studies were supported by parallel size and zeta potential measurements. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：80 / 85
页数：6
相关论文
共 45 条
[1]   The efficacy of aerosol treatment with non-ionic surfactant vesicles containing amphotericin B in rodent models of leishmaniasis and pulmonary aspergillosis infection [J].
Alsaadi, M. ;
Italia, J. L. ;
Mullen, A. B. ;
Kumar, M. N. V. Ravi ;
Candlish, A. A. ;
Williams, R. A. M. ;
Shaw, C. D. ;
Al Gawhari, F. ;
Coombs, G. H. ;
Wiese, M. ;
Thomson, A. H. ;
Puig-Sellart, M. ;
Wallace, J. ;
Sharp, A. ;
Wheeler, L. ;
Warn, P. ;
Carter, K. C. .
JOURNAL OF CONTROLLED RELEASE, 2012, 160 (03) :685-691
[2]   Immune responses of Leishmania donovani infected BALB/c mice following treatment with free and vesicular sodium stibogluconate formulations [J].
Banduwardene, R ;
Mullen, AB ;
Carter, KC .
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, 1997, 19 (04) :195-203
[3]  
Barbeau J., 2011, J DRUG DELIV, V2011, P1
[4]   Characterization of Niosomes Prepared With Various Nonionic Surfactants for Paclitaxel Oral Delivery [J].
Bayindir, Zerrin Sezgin ;
Yuksel, Nilufer .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2010, 99 (04) :2049-2060
[5]   Comparative assessment of a DNA and protein Leishmania donovani gamma glutamyl cysteine synthetase vaccine to cross-protect against murine cutaneous leishmaniasis caused by L. major or L. mexicana infection [J].
Campbell, S. A. ;
Alawa, J. ;
Doro, B. ;
Henriquez, F. L. ;
Roberts, C. W. ;
Nok, A. ;
Alawa, C. B. I. ;
Alsaadi, M. ;
Mullen, A. B. ;
Carter, K. C. .
VACCINE, 2012, 30 (07) :1357-1363
[6]   Clinical development of liposome-based drugs: formulation, characterization, and therapeutic efficacy [J].
Chang, Hsin-I ;
Yeh, Ming-Kung .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2012, 7 :49-60
[7]   Mechanism of response enhancement in evaporative light scattering detection with the addition of triethylamine and formic acid [J].
Deschamps, FS ;
Baillet, A ;
Chaminade, P .
ANALYST, 2002, 127 (01) :35-41
[8]   Assessment of the retention properties of poly(vinyl alcohol) stationary phase for lipid class profiling in liquid chromatography [J].
Deschamps, FS ;
Chaminade, P ;
Ferrier, D ;
Baillet, A .
JOURNAL OF CHROMATOGRAPHY A, 2001, 928 (02) :127-137
[9]   Response enhancement for the evaporative light scattering detection for the analysis of lipid classes and molecular species [J].
Deschamps, FS ;
Gaudin, K ;
Lesellier, E ;
Tchapla, A ;
Ferrier, D ;
Baillet, A ;
Chaminade, P .
CHROMATOGRAPHIA, 2001, 54 (9-10) :607-611
[10]   Novel pH-sensitive non-ionic surfactant vesicles: comparison between Tween 21 and Tween 20 [J].
Di Marzio, Luisa ;
Marianecci, Carlotta ;
Petrone, Mariadea ;
Rinaldi, Federica ;
Carafa, Maria .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2011, 82 (01) :18-24
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