In vitro isolation of Neospora caninum from a stillborn calf in the UK

被引：28
作者：
Davison, HC
Guy, F
Trees, AJ
Ryce, C
Ellis, JT
Otter, A
Jeffrey, M
Simpson, VR
Holt, JJ
机构：
[1] Univ Liverpool, Liverpool Sch Trop Med, Fac Vet Sci, Liverpool L3 5QA, Merseyside, England
[2] Univ Technol, Sydney, Gore Hill, Australia
[3] Vet Invest Ctr, Vet Labs Agcy, Carmarthen SA31 3EZ, Dyfed, Wales
[4] Lasswade Vet Lab, Penicuik EH26 0PZ, Midlothian, Scotland
[5] Vet Invest Ctr, Vet Labs Agcy, Truro TR4 9AD, Cornwall, England
[6] Vet Surg, Camelford PL32 9RX, Cornwall, England
来源：
RESEARCH IN VETERINARY SCIENCE | 1999年 / 67卷 / 01期
关键词：
D O I：
10.1053/rvsc.1998.0272
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Neospora caninum was isolated in Vero cell culture from the brain of a stillborn calf. This isolate (designated NC-LiVB1) is the first to be obtained from cattle in the United Kingdom and was confirmed as N. caninum by immunofluorescence with specific antibodies and by internal transcribed spacer 1 (ITS 1) sequence analysis. Differences were found between NC-LiVB1, other bovine isolates and canine isolates of N. caninum and closely related protozoal parasites, using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) techniques.
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页码：103 / 105
页数：3
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