Regulation of the plasma amino acid profile by leucine via the system L amino acid transporter

被引：9

作者：

Zhen, Hongmin ^{[1
]}

Nakamura, Koichi ^{[1
]}

Kitaura, Yasuyuki ^{[1
]}

Kadota, Yoshihiro ^{[1
]}

Ishikawa, Takuya ^{[1
]}

Kondo, Yusuke ^{[1
]}

Xu, Minjun ^{[1
]}

Shimomura, Yoshiharu ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Mol Biosci, Lab Nutr Biochem, Nagoya, Aichi 4648601, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2015年 / 79卷 / 12期

关键词：

branched-chain amino acids; BCH; rapamycin; plasma amino acid profile; CHAIN; IDENTIFICATION; PROTEIN;

D O I：

10.1080/09168451.2015.1060845

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasma concentrations of amino acids reflect the intracellular amino acid pool in mammals. However, the regulatory mechanism requires clarification. In this study, we examined the effect of leucine administration on plasma amino acid profiles in mice with and without the treatment of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) or rapamycin as an inhibitor of system L or mammalian target of rapamycin complex 1, respectively. The elevation of plasma leucine concentration after leucine administration was associated with a significant decrease in the plasma concentrations of isoleucine, valine, methionine, phenylalanine, and tyrosine; BCH treatment almost completely blocked the leucine-induced decrease in plasma amino acid concentrations. Rapamycin treatment had much less effects on the actions of leucine than BCH treatment. These results suggest that leucine regulates the plasma concentrations of branched-chain amino acids, methionine, phenylalanine, and tyrosine, and that system L amino acid transporters are involved in the leucine action.

引用

页码：2057 / 2062

页数：6

共 27 条

[1] Identification of a novel system L amino acid transporter structurally distinct from heterodimeric amino acid transporters [J].