Production and optimization of xylooligosaccharides from corncob by Bacillus aerophilus KGJ2 xylanase and its antioxidant potential

被引：70
|
作者：
Gowdhaman, D. [1 ]
Ponnusami, V. [1 ]
机构：
[1] SASTRA Univ, Sch Chem & Biotechnol, Bioproc Intensificat Lab, Thanjavur 613401, Tamil Nadu, India
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 79卷
关键词：
Corncob; Xylooligosaccharides (XOS); Antioxidant activity; ENZYMATIC PRODUCTION; FUNGAL XYLANASE; AUTOHYDROLYSIS; OLIGOSACCHARIDES; MANUFACTURE;
D O I：
10.1016/j.ijbiomac.2015.05.046
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of the present study is to produce xylooligosaccharides (XOS) from corncob xylan. The xylan was extracted from corncob using methods like dilute acid, dilute alkali and sodium hypochlorite treatment. Corncob xylan extracted using alkali was characterized by FT-IR and TG-DSC. The extracted xylan was subjected to enzymatic hydrolysis using Bacillus aerophilus KGJ2 xylanase for XOS production. To increase the yield of XOS, the effects of various process parameters like substrate concentration, reaction time, and enzyme concentration on XOS production were investigated. XOS prepared was characterized by HPTLC. Anti oxidant potential of produced XOS was evaluated and the DPPH assay showed that XOS possessed concentration dependent free radical scavenging activity. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：595 / 600
页数：6
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