Hepatoprotective principles from the flowers of Tilia argentea (Linden):: Structure requirements of tiliroside and mechanisms of action

被引：110
作者：
Matsuda, H
Ninomiya, K
Shimoda, H
Yoshikawa, M [1 ]
机构：
[1] Kyoto Pharmaceut Univ, Yamashina Ku, Kyoto 6078412, Japan
[2] Morishita Jintan Co Ltd, Chuo Ku, Osaka 5408566, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2002年 / 10卷 / 03期
关键词：
D O I：
10.1016/S0968-0896(01)00321-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The methanolic extract from the flowers of Tilia argentea (linden) was found to show a hepatoprotective effect against D-galactosamine (D-GalN)/lipopolysaccharide (LPS)-induced liver injury in mice. By bioassay-guided separation using in vitro D-GalN-induced damage to hepatocytes, five flavonol glycosides were isolated as the hepatoprotective constituents of the methanolic extract. Tiliroside. the principal flavonol glycoside. strongly inhibited serum GPT and GOT elevations at doses of 25-100 mg/kg (p.o.) in D-GalN/LPS-treated mice. By comparing the inhibitory effects of tiliroside with those of its components alone. the kaempferol 3-O-beta-D-glucopyranoside moiety was found to be essential for the activity, and its effect was suggested to depend on the inhibition of tumor necrosis factor-alpha (TNF-alpha) production. decreased sensitivity of hepatocytes to TNF-alpha. and on the protection of hepatocytes against D-GalN. (C) 2002 Elsevier Science Ltd. All rights reserved.
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页码：707 / 712
页数：6
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