Molecular markers for chloroquine-resistant Plasmodium falciparum malaria in Thailand and Laos

被引:15
作者
Labbé, AC
Bualombai, P
Pillai, DR
Zhong, KYJ
Vanisaveth, V
Hongvanthong, B
Looareesuwan, S
Kain, KC
机构
[1] Univ Toronto, Dept Med, Div Infect Dis, Trop Dis Unit, Toronto, ON, Canada
[2] Minist Publ Hlth, Dept Communicable Dis Control, Malaria Div, Nonthaburi, Thailand
[3] Inst Malariol Parasitol & Entomol, Viangchan, Laos
[4] Mahidol Univ, Fac Trop Med, Hosp Trop Dis, Bangkok 10700, Thailand
来源
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY | 2001年 / 95卷 / 08期
关键词
D O I
10.1080/00034980120103414
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Chloroquine-resistant Plasmodium falciparum is well documented in Thailand. Laos, however, continues to use chloroquine (CQ) as the first-line therapy for the treatment of P. falciparum malaria. The objective of the present study was to determine the prevalence, in these two areas, of the cg2, pfmdr1 and pfcrt allelic types that have previously been associated with CQ resistance. Isolates of P. falciparum were collected from participants in ongoing treatment studies conducted in Thailand (near the Thai-Cambodian border) and in Laos (Vang Vieng district). The pfmdr1 and pfcrt alleles were characterized by PCR-RFLP and mutations in cg2 were characterized by PCR and single-stranded-conformation-polymorphism (SSCP) electrophoresis. Eight (32%) of the 25 Laotian isolates but only one (4%) of the 25 Thai isolates were found to contain the pfmdr1 mutation N86Y (P = 0.02). In contrast, the cg2 polymorphisms previously associated with CQ resistance were present in only 10 of the isolates from Laos but 24 of those from Thailand (40% v. 96%; P<0.001). All the samples from both countries contained the pfcrt K76T mutant allele reported to confer resistance to CQ. The results may indicate that drug pressure for the maintenance of the pfmdr1 and cg2 alleles varies in intensity in the Thai and Laotian study areas, probably reflecting differences in the national malaria-treatment policies of Thailand and Laos.
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页码:781 / 788
页数:8
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