The detection of autoantibodies to ATP-binding cassette transporter A1 and its role in the pathogenesis of atherosclerosis in patients with systemic lupus erythematosus

被引:8
作者
Zeng, Ting [1 ]
Li, Shu-Jie [1 ]
Ao, Wen [1 ]
Zheng, Hui [1 ]
Wu, Feng-Xia [1 ]
Chen, Yi [1 ]
Yang, Cheng-De [1 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Dept Rheumatol, Shanghai 200001, Peoples R China
基金
中国国家自然科学基金;
关键词
Systemic lupus erythematosus; ATP-binding cassette transporter A1; Atherosclerosis; CHOLESTEROL EFFLUX; ENDOTHELIAL-CELLS; TANGIER-DISEASE; RISK-FACTORS; ABCA1; ANTIBODIES; WOMEN; ASSOCIATION; DEGRADATION; PREVALENCE;
D O I
10.1016/j.clinbiochem.2012.06.009
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Objectives: To investigate the prevalence of autoantibodies against ATP-binding cassette transporter A1 (ABCA1) in SLE patients, and evaluate the association between anti-ABCA1 autoantibodies and atherosclerosis in SLE. Design and methods: The sera of 75 SLE patients and 75 healthy controls were tested by immunoblotting. Then, we examined the effect of anti-ABCA1 autoantibodies on cholesterol efflux in vitro. Results: The prevalence of anti-ABCA1 antibodies in SLE patients was significantly higher than the controls (p < 0.05). The prevalence in the SLE-plaque group was higher than that in the SLE-non-plaque group (p < 0.05). The IgG purified from anti-ABCA1-antibody positive sera can inhibit cellular cholesterol efflux from THP-1 cells in vitro with a significantly higher inhibition ratio than that of the healthy controls. Conclusions: Our observations suggest that anti-ABCA1 autoantibodies are involved in the pathogenesis of lupus atherosclerosis and that autoantibodies against ABCA1 may act as biomarkers for atherosclerosis in SLE. (c) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:1342 / 1346
页数:5
相关论文
共 33 条
  • [1] ABCA1 expression in carotid atherosclerotic plaques
    Albrecht, C
    Soumian, S
    Amey, JS
    Sardini, A
    Higgins, CF
    Davies, AH
    Gibbs, RGJ
    [J]. STROKE, 2004, 35 (12) : 2801 - 2806
  • [2] Helical apolipoproteins stabilize ATP-binding cassette transporter A1 by protecting it from thiol protease-mediated degradation
    Arakawa, R
    Yokoyama, S
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (25) : 22426 - 22429
  • [3] ABCA1: at the nexus of cholesterol, HDL and atherosclerosis
    Attie, Alan D.
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 2007, 32 (04) : 172 - 179
  • [4] DERIVATION OF THE SLEDAI - A DISEASE-ACTIVITY INDEX FOR LUPUS PATIENTS
    BOMBARDIER, C
    GLADMAN, DD
    UROWITZ, MB
    CARON, D
    CHANG, CH
    [J]. ARTHRITIS AND RHEUMATISM, 1992, 35 (06): : 630 - 640
  • [5] Frequency of antibodies to the cholesterol transport protein apolipoprotein A1 in patients with SLE
    Dinu, AR
    Merrill, JT
    Shen, C
    Antonov, IV
    Myones, BL
    Lahita, RG
    [J]. LUPUS, 1998, 7 (05) : 355 - 360
  • [6] Association of biological markers of activity of systemic lupus erythematosus with levels of anti-oxidized low-density lipoprotein antibodies
    Gómez-Zumaquero, JM
    Tinahones, FJ
    De Ramón, E
    Camps, M
    Garrido, L
    Soriguer, FJ
    [J]. RHEUMATOLOGY, 2004, 43 (04) : 510 - 513
  • [7] Atherosclerosis and lupus
    Gorman, C
    Isenberg, D
    [J]. RHEUMATOLOGY, 2004, 43 (08) : 943 - 945
  • [8] Immunology of ischemic vascular disease: plaque to attack
    Hallenbeck, JM
    Hansson, GK
    Becker, KJ
    [J]. TRENDS IN IMMUNOLOGY, 2005, 26 (10) : 550 - 556
  • [9] Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus
    Hochberg, MC
    [J]. ARTHRITIS AND RHEUMATISM, 1997, 40 (09): : 1725 - 1725
  • [10] CAROTID-ARTERY INTIMAL-MEDIAL THICKNESS DISTRIBUTION IN GENERAL POPULATIONS AS EVALUATED BY B-MODE ULTRASOUND
    HOWARD, G
    SHARRETT, AR
    HEISS, G
    EVANS, GW
    CHAMBLESS, LE
    RILEY, WA
    BURKE, GL
    [J]. STROKE, 1993, 24 (09) : 1297 - 1304