Effects of TGF-β1 and hydrostatic pressure on meniscus cell-seeded scaffolds

The combinatorial effects of TGF-beta 1 and hydrostatic pressure (HP) were investigated oil meniscus cell-seeded PLLA constructs using a two-phase sequential study. The objective was to identify potentially synergistic effects of these stimuli toward enhancing the biomechanical and compositional characteristics of the engineered constructs. In Phase 1, the effects of TGF-beta 1 were examined oil the ability of meniscus cells to produce ECM. In Phase 11, meniscus cell-seeded PLLA constructs were cultured for 4 wks with a combination of TGF-beta 1 and HP (10 MPa, 0 Hz or 10 MPa, 0.1 Hz). TGF-beta 1 Was found to increase collagen and GAG deposition in the scaffolds 15-fold and 8-fold, respectively, in Phase 1. In Phase 11, the combination of TGF-beta 1 and 10 MPa, 0 Hz HP resulted in 4-fold higher collagen deposition (additive increase), 3-fold higher GAG deposition and enhanced compressive properties (additive and synergistic increases), when Compared to the unpressurized no growth factor culture control. Though significant correlations were observed between the compressive properties (moduli and viscosity), and the GAG and collagen content of the Constructs, the correlations were stronger with collagen. This Study provides robust evidence that growth factors and HP call be used Successfully in combination to enhance the functional properties of ill vitro engineered knee meniscus Constructs. (C) 2008 Elsevier Ltd. All rights reserved.