Analysis of Salvinorin A in urine using microextraction in packed syringe and GC-MS/MS

被引:0
作者
Moreno, Ivo [1 ]
da Fonseca, Beatriz [1 ]
Oppolzer, David [1 ]
Martinho, Ana [1 ]
Barroso, Mario [2 ]
Cruz, Angelines [3 ]
Queiroz, Joao Antonio [1 ]
Gallardo, Eugenia [1 ]
机构
[1] Univ Beira Interior, CICS UBI Ctr Invest Ciencias Saude, P-6201556 Covilha, Portugal
[2] Inst Nacl Med Legal Delegacao Sul, P-1150219 Lisbon, Portugal
[3] Univ Santiago de Compostela, Inst Ciencias Forenses Luis Concheiro, Serv Toxicol Forense, Santiago De Compostela 15782, Spain
关键词
GAS CHROMATOGRAPHY/MASS SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; PIPERAZINE-TYPE STIMULANTS; THIN-LAYER-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; SALVIA-DIVINORUM; PLASMA SAMPLES; SORBENT; QUANTIFICATION; IONIZATION;
D O I
10.4155/BIO.13.18
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: The aim of this work was to develop and validate a method for the determination of Salvinorin A in human urine using microextraction by packed sorbent (MEPS) and GC MS/MS. Results: The technique uses a sample volume as low as 0.2 ml, and the analyte was extracted using a C18 sorbent. The method showed to be linear between 20 and 1000 ng/ml and presented a LOD of 5 ng/ml. Intra- and inter-day precision and accuracy were acceptable. Absolute recoveries ranged from 71 to 80%. Conclusion: GC MS/MS with MEPS demonstrated to be a fast and simple procedure for the quantification of Salvinorin A in urine. This is the first time that GC MS/MS with MEPS was used for the determination of this compound in biological fluids. Furthermore, the device could be reused for up to 80 extractions, which accounted for a lower cost of analysis.
引用
收藏
页码:661 / 668
页数:8
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