MicroRNA-107 Inhibits U87 Glioma Stem Cells Growth and Invasion

被引：48

作者：

Chen, Lei ^{[1
]}

Chen, Xiang-rong ^{[2
,3
]}

Chen, Fan-fan ^{[2
,4
]}

Liu, Yi ^{[1
]}

Li, Peng ^{[1
]}

Zhang, Run ^{[1
]}

Yan, Ke ^{[1
]}

Yi, Yong-jun ^{[1
]}

Xu, Zhi-min ^{[1
]}

Jiang, Xiao-Dan ^{[1
]}

机构：

[1] Southern Med Univ, Natl Key Clin Specialty, Guangdong Prov Key Lab Brain Funct Repair & Regen, Zhujiang Hosp,Dept Neurosurg,Neurosurg Inst Guang, Guangzhou 510282, Guangdong, Peoples R China

[2] Southern Med Univ, Zhujiang Hosp, Dept Neurosurg, Guangzhou 510282, Guangdong, Peoples R China

[3] Fujian Med Univ, Affiliated Hosp 2, Dept Neurosurg, Quanzhou 362000, Peoples R China

[4] Guangzhou First Peoples Hosp, Dept Neurosurg, Guangzhou 510180, Guangdong, Peoples R China

来源：

CELLULAR AND MOLECULAR NEUROBIOLOGY | 2013年 / 33卷 / 05期

关键词：

miR-107; Glioma stem cells; Proliferation; Invasiveness; Notch2; TENASCIN-C; BRAIN-TUMORS; IDENTIFICATION; EXPRESSION; MIGRATION; PATHOLOGY; GENE;

D O I：

10.1007/s10571-013-9927-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Glioma stem cells (GSCs) are thought to be critical for resistance to radiotherapy and chemotherapy and for tumor recurrence after surgery in glioma patients. Identification of new therapeutic strategies that can target GSCs may thus be critical for improving patient survival. MicroRNAs (miRNAs) are small non-coding RNAs that function as tumor suppressors or oncogenes. In this study, we confirmed that miR-107 was down-regulated in GSCs. To investigate the role of miR-107 in tumorigenesis of GSCs, a lentiviral vector over-expressing miR-107 in U87GSCs was constructed. We found that over-expression of miR-107 suppressed proliferation and down-regulated Notch2 protein and stem cell marker (CD133 and Nestin) expression in U87GSCs. Furthermore, enhanced miR-107 expression significantly inhibited U87GSC invasion and reduced matrix metalloproteinase-12 expression. miR-107 also suppressed U87GSCs xenograft growth in vivo. These findings suggest that miR-107 is involved in U87GSCs growth and invasion and may provide a potential therapeutic target for glioma treatment.

引用

页码：651 / 657

页数：7

共 32 条

[1] Notch signaling: Cell fate control and signal integration in development [J].