The nuclear ceramide/diacylglycerol balance depends on the physiological state of thyroid cells and changes during UV-C radiation-induced apoptosis

被引：27
作者：
Albi, E. [1 ]
Cataldi, S. [1 ]
Rossi, G. [1 ]
Magni, M. Viola [1 ]
Toller, M. [2 ]
Casani, S. [2 ]
Perrella, G. [2 ]
机构：
[1] Monteluce Policlin, Physiopathol Sect, Dept Clin & Expt Med, I-06100 Perugia, Italy
[2] Univ Udine, Dept Expt & Clin Pathol & Med, I-33100 Udine, Italy
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2008年 / 478卷 / 01期
关键词：
apoptosis; ceramide; neutral-sphingomyelinase; nucleus; reverse-sphingomyelin-synthase; sphingomyelin-synthase; thyroid cell; ultraviolet rays;
D O I：
10.1016/j.abb.2008.07.018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Intranuclear lipid metabolism modifications in relation to cell proliferation and/or apoptosis were demonstrated in hepatocytes. The aim of this study was to establish whether nuclear lipid metabolites influence cell function in different experimental models using a rat thyroid cell line (FRTL-5) treated with UV-C radiation. After UV-C irradiation cells proliferate and undergo apoptosis in the presence of thyrotropin, are quiescent and resistant to radiation-induced apoptosis in its absence and finally are proapoptotic for nutrition withdrawal. In nuclei purified from proliferating cells, irradiation stimulates neutral-sphingomyelinase activity and inhibits sphingomyelin-synthase, phosphatidylcholine-specific phospholipase C and phosphatidylinositol-specific phospholipase C activity with a consequent increase in the ceramide/diacylglycerol ratio. This effect is marked in proapoptotic cell nuclei and low in quiescent cell nuclei. In conclusion, UV-C radiation induces apoptosis, modifying nuclear lipid metabolism in relation to the physiological state of cells. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：52 / 58
页数：7
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