Identification of Six Small Heat Shock Protein Genes in Ostrinia furnacalis (Lepidoptera: Pyralidae) and Analysis of Their Expression Patterns in Response to Environmental Stressors

被引:8
作者
Ruan, Hong-Yun [1 ]
Meng, Jian-Yu [2 ]
Yang, Chang-Li [1 ]
Zhou, Lv [1 ]
Zhang, Chang-Yu [1 ,2 ]
机构
[1] Guizhou Univ, Inst Entomol, Guizhou Prov Key Lab Agr Pest Management Mt Reg, Guiyang 550025, Guizhou, Peoples R China
[2] Guizhou Tobacco Sci Res Inst, Guiyang 550081, Guizhou, Peoples R China
基金
国家重点研发计划;
关键词
Ostrinia furnacalis; small heat shock protein; extreme temperature; UV-A; OXIDATIVE STRESS; TEMPORAL EXPRESSION; CHILL COMA; RADIATION; CLONING; TEMPERATURE; DIPTERA; EVOLUTIONARY; ULTRAVIOLET; DIVERSITY;
D O I
10.1093/jisesa/ieac069
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Ostrinia furnacalis (Guenee) is a major insect pest in maize production that is highly adaptable to the environment. Small heat shock proteins (sHsps) are a class of chaperone proteins that play an important role in insect responses to various environmental stresses. The present study aimed to clarify the responses of six O. furnacalis sHsps to environmental stressors. In particular, we cloned six sHsp genes, namely, OfHsp24.2, OfHsp21.3, OfHsp20.7, OfHsp21.8, OfHsp29.7, and OfHsp19.9, from O. furnacalis. The putative proteins encoded by these genes contained a typical alpha-crystallin domain. Real-time quantitative polymerase chain reaction was used to analyze the differences in the expression of these genes at different developmental stages, in different tissues of male and female adults, and in O. furnacalis under UV-A and extreme temperature stresses. The six OfsHsp genes were expressed at significantly different levels based on the developmental stage and tissue type in male and female adults. Furthermore, all OfsHsp genes were significantly upregulated in both male and female adults under extreme temperature and UV-A stresses. Thus, O. furnacalis OfsHsp genes play important and unique regulatory roles in the developmental stages of the insect and in response to various environmental stressors.
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页数:13
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