Scanning electron microscopy of a blister roof in dystrophic epidermolysis bullosa

被引：1

作者：

de Almeida, Hiram Larangeira, Jr. ^{[1
,2
]}

Monteiro, Luciane ^{[3
]}

Marques e Silva, Ricardo ^{[4
]}

Rocha, Nara Moreira ^{[5
]}

Scheffer, Hans ^{[6
]}

机构：

[1] Univ Fed Pelotas, Pelotas, RS, Brazil

[2] Catholic Univ Pelotas UFPel, Pelotas, RS, Brazil

[3] Catholic Univ Pelotas UFPel, Postgrad Program Hlth, Pelotas, RS, Brazil

[4] Fed Univ Pelotas UFPel, Lab Electron Microscopy, Pelotas, RS, Brazil

[5] Empresa Brasileira Pesquisa Agr EMBRAPA CPA CT, Electron Microscopy Lab, Pelotas, RS, Brazil

[6] Radboud Univ Nijmegen, Med Ctr, DNA Diagnost Div, Dept Human Genet, NL-6525 ED Nijmegen, Netherlands

来源：

ANAIS BRASILEIROS DE DERMATOLOGIA | 2013年 / 88卷 / 06期

关键词：

Basement membrane; Epidermolysis bullosa dystrophica; Microscopy; electron; scanning; Skin diseases; vesiculobullous; MUTATION;

D O I：

10.1590/abd1806-4841.20131743

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

In dystrophic epidermolysis bullosa the genetic defect of anchoring fibrils leads to cleavage beneath the basement membrane, with its consequent loss. We performed scanning electron microscopy of an inverted blister roof of a case of dystrophic epidermolysis bullosa, confirmed by immunomapping and gene sequencing. With a magnification of 2000 times a net attached to the blister roof could be easily identified. This net was composed of intertwined flat fibers. With higher magnifications, different fiber sizes could be observed, some thin fibers measuring around 80 nm and thicker ones measuring between 200 and 300 nm.

引用

页码：966 / 968

页数：3

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