Enhancement of metal ion adsorption capacity of Saccharomyces cerevisiae's cells by using disruption method

Herein disruption of S. cerevisiae's cells by bead mills as a novel treatment to improve adsorption capacity for Cu(II) ions was studied. The experiments were done in batch process by altering parameters like pH, drying temperature, biosorbant content, Cu initial concentration and reaction time. Adsorption capacity was achieved by RSM (response surface methodology) 10.46 mg/g and 15.33 mg/g in intact and disrupted biomass respectively. The optimum biomass drying temperature was obtained in 180 degrees C. Langmuir isotherm showed best fitting and pseudo-second-order kinetic revealed chemical adsorption. Comparison FTIR analysis of intact biomass, disrupted biomass before adsorption and after Cu(II) loading, indicated that although carboxylic acid and hydroxyl groups were common contributors in adsorption process for both intact and disrupted biomass, major role attributed to thiol groups oxidated during biomass disruption and formed disulfide bonds which have more tendency to Cu(II) adsorption. SEM analysis used to compare biosorbant morphology before and after cell disruption. (C) 2013 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.