The composition of essential oils from the flowers, leaves, and stems of Wedelia urticifolia were analysed. alpha-Pinene (19.80%) and d-limonene (17.31%) were the most abundant compounds in flower oils, while a-pinene (15.57%), d-limonene (11.19%), a-phellandrene (9.69%), gamma-terpinene (9.01%), and germacrene D (9.68%) were the main constituents of leaf oil. In stems oils, the major compounds were D-limonene (18.11%), gamma-terpinene (13.89%), germacrene D (12.80%), and alpha-pinene (9.21%). Oils from flowers, leaves, and stems exhibited antimicrobial activity against a variety of bacteria, yeasts, and moulds as indicated by the diameter of zones of inhibition (flower oil: 20.8-23.2 mm, leaf oil: 19.4-21.2 mm, stem oil: 18.6-20.6 mm) and their respective MIC values (flower oil: 6.25-25.0 mu g/mL, leaf oil: 12.5-50.0 mu g/mL, stem oil: 25.0-50.0 mu g/mL).