Determination of Berberine and Berberrubine in Rabbit Plasma by Capillary Electrophoresis with Amperometric Detection and its Application to Pharmacokinetic Study

被引:0
作者
Deng, Guang-Hui [1 ]
Wang, Hui [1 ]
Gao, Jin [1 ]
Wang, Shiwei [1 ]
机构
[1] Guangxi Univ, Coll Chem & Ecoengn, Guangxi Univ Nationalities, Key Lab Chem & Biol Transforming Proc, Nanning 530000, Peoples R China
来源
LATIN AMERICAN JOURNAL OF PHARMACY | 2012年 / 31卷 / 05期
关键词
Amperometric detection; Berberine; Berberrubine; Capillary electrophoresis; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLUORESCENCE DETECTION; CHINESE MEDICINE; PALMATINE; SAMPLES; URINE; BILE; MS;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
A simple and selective method based on capillary electrophoresis with amperometric detection has been developed for the separation and determination of berberine (BB) and berberrubine (BBR) in rabbit plasma. The working electrode was a 150 pm diameter platinum microelectrode at a detection potential of +1.1 V (versus Ag/AgCl). The analytes could be well separated within 6 min in a 50 cm length fused-silica capillary at a separation voltage of 15 kV in a 50 mmol/L phosphate buffer (pH 6.0). Calibration curve was linear over the range of 0.002-20 mu g/mL. The detection limits (S/N = 3) were 1.0 and 0.8 ng/mL for BB and BBR, respectively. Relative standard deviations (R.S.D.) of not more than 6.0 % were obtained for both migration times and electrophoretic peak area. The proposed method was successfully used to analyze the drugs in samples of rabbit plasma for pharmacokinetic study.
引用
收藏
页码:665 / 670
页数:6
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