Identification and characterization of antioxidative peptides derived from simulated in vitro gastrointestinal digestion of walnut meal proteins

被引：98
作者：
Feng, Li [1 ]
Peng, Fei [1 ]
Wang, Xiaojing [1 ]
Li, Mei [1 ]
Lei, Hongjie [1 ]
Xu, Huaide [1 ]
机构：
[1] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China
来源：
FOOD RESEARCH INTERNATIONAL | 2019年 / 116卷
基金：
中国国家自然科学基金;
关键词：
Walnut meal protein; Antioxidant peptides; Gastrointestinal digestion; Radical scavenging activity; Cell viability; OXIDATIVE STRESS; PURIFICATION; HYDROLYSATE; ABSORPTION; CAPACITY;
D O I：
10.1016/j.foodres.2018.08.068
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The aim of this study was to isolate and identify antioxidant peptides from defatted walnut meal proteins hydrolysates (DWMPH) prepared by simulated gastrointestinal digestion, and to evaluate the protective effect of the selected antioxidant peptides against oxidative damage to SH-SY5Y cells. The DWMPH were purified by ultrafiltration, gel filtration chromatography and RP-HPLC successively, and finally six major peptides were identified as VRN, NPAN, AHSVGP, SSE, TY and SGGY by UPLC-QTOF-MS. TY and SGGY displayed stronger ABTS radical scavenging activity and higher ORAC value in comparison with other four peptides. Furthermore, 0.5 mg/mL SGGY exhibited significantly protective effect on SH-SY5Y cells against oxidative damage (p < .01). Therefore, it was suggested that walnut meal protein could serve as a potential source of antioxidative peptides through gastrointestinal digestion.
引用
收藏
页码：518 / 526
页数：9
相关论文
共 34 条
[1]   Ultrasound-mediated blood-brain barrier disruption for targeted drug delivery in the central nervous system [J].
Aryal, Muna ;
Arvanitis, Costas D. ;
Alexander, Phillip M. ;
McDannold, Nathan .
ADVANCED DRUG DELIVERY REVIEWS, 2014, 72 :94-109
[2]   High molecular weight melanoidins from coffee brew [J].
Bekedam, E. Koen ;
Schols, Henk A. ;
Van Boekel, Martinus A. J. S. ;
Smit, Gerrit .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (20) :7658-7666
[3]   Identification of antioxidative peptides from defatted walnut meal hydrolysate with potential for improving learning and memory [J].
Chen, Huiping ;
Zhao, Mouming ;
Lin, Lianzhu ;
Wang, Jufang ;
Sun-Waterhouse, Dongxiao ;
Dong, Yi ;
Zhuang, Mingzhu ;
Su, Guowan .
FOOD RESEARCH INTERNATIONAL, 2015, 78 :216-223
[4]   Purification and identification of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates [J].
Chen, Ning ;
Yang, Hongmei ;
Sun, Yi ;
Niu, Jun ;
Liu, Shuying .
PEPTIDES, 2012, 38 (02) :344-349
[5]   Purification and identification of three novel antioxidant peptides from protein hydrolysate of bluefin leatherjacket (Navodon septentrionalis) skin [J].
Chi, Chang-Feng ;
Wang, Bin ;
Hu, Fa-Yuan ;
Wang, Yu-Mei ;
Zhang, Bin ;
Deng, Shang-Gui ;
Wu, Chang-Wen .
FOOD RESEARCH INTERNATIONAL, 2015, 73 :124-129
[6]   Protein breakdown and release of antioxidant peptides during simulated gastrointestinal digestion and the absorption by everted intestinal sac of rapeseed proteins [J].
Dai, Chunhua ;
Zhang, Weiwei ;
He, Ronghai ;
Xiong, Feng ;
Ma, Haile .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2017, 86 :424-429
[7]   Extending applicability of the oxygen radical absorbance capacity (ORAC-fluorescein) assay [J].
Dávalos, A ;
Gómez-Cordovés, C ;
Bartolomé, B .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (01) :48-54
[8]   Walnuts reduce aortic ET-1 mRNA levels in hamsters fed a high-fat, atherogenic diet [J].
Davis, P ;
Valacchi, G ;
Pagnin, E ;
Shao, QM ;
Gross, HB ;
Calo, L ;
Yokoyama, W .
JOURNAL OF NUTRITION, 2006, 136 (02) :428-432
[9]   Purification and characterization of a natural antioxidant peptide from fertilized eggs [J].
Duan, Xiang ;
Ocen, Denis ;
Wu, Fengfeng ;
Li, Mei ;
Yang, Na ;
Xu, Jin ;
Chen, Haiying ;
Huang, Liqun ;
Jin, Zhengyu ;
Xu, Xueming .
FOOD RESEARCH INTERNATIONAL, 2014, 56 :18-24
[10]   Insights into digestion and absorption of major nutrients in humans [J].
Goodman, Barbara E. .
ADVANCES IN PHYSIOLOGY EDUCATION, 2010, 34 (02) :44-53
1234