Molecular characterization of a 2-Cys peroxiredoxin induced by abiotic stress in mungbean

被引：11

作者：

Cho, Chang-Woo ^{[1
]}

Chung, Eunsook ^{[1
]}

Heo, Jee-Eun ^{[1
]}

So, Hyun-Ah ^{[1
]}

Choi, Hong-Kyu ^{[1
]}

Kim, Doh Hoon ^{[1
]}

Chung, Young Soo ^{[1
]}

Chae, Ho Zoon ^{[2
]}

Lee, Jai-Heon ^{[1
]}

机构：

[1] Dong A Univ, Ctr Silver Bio Industrializat BK21, Coll Nat Resources & Life Sci, Pusan 604714, South Korea

[2] Chonnam Natinal Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2012年 / 108卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

Abiotic stress; MFO; Oxidative stress; Plastid; 2-Cys peroxiredoxin; Vigna radiata; ARABIDOPSIS-THALIANA; HYDROGEN-PEROXIDE; THIOREDOXIN PEROXIDASE; REDOX REGULATION; LOW-TEMPERATURE; CHLOROPLAST; EXPRESSION; PROTEINS; DETOXIFICATION; TRANSFORMATION;

D O I：

10.1007/s11240-011-0061-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A mungbean low temperature-inducible VrPrx1 encoding 2-Cys peroxiredoxin (2-Cys Prx) was cloned by subtractive suppression hybridization. The deduced VrPrx1 amino acid sequence showed highest sequence homology to 2-Cys Prxs of Phaseolus vulgaris (95%), Pisum sativum (89%), and Arabidopsis thaliana (87%). VrPrx1 RNA and protein levels were increased by low temperature, hydrogen peroxide (H2O2), and wounding but decreased by high salinity, drought, and exogenous abscisic acid. Recombinant His-tagged VrPrx1 recombinant protein protected DNA and glutamine synthetase activity from degradation via the thiol/Fe(III) oxygen mixed-function oxidation system, and exhibited peroxidase activity to H2O2 in the presence of the reducing agent dithiothreitol (DTT) in vitro. The oxidized dimers and oligomers of the VrPrx1 recombinant protein were reduced to monomers by DTT or thioredoxin. Subcellular localization studies confirmed that VrPrx1-GFP was targeted to the plastid. To evaluate the function of VrPrx1 in planta, the antioxidant activities and photosynthetic efficiency were investigated in VrPrx1-overexpressing Arabidopsis plants. VrPrx1 ectopic expression conferred improved photosynthetic efficiency under oxidative stress conditions. Hence, mungbean VrPrx1 may play an important role in protecting the photosynthetic apparatus against oxidative and abiotic stress conditions.

引用

页码：473 / 484

页数：12

共 56 条

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