Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)

被引：19
|
作者：
Nkere, Chukwuemeka K. [1 ,2 ,3 ]
Oyekanmi, Joshua O. [1 ]
Silva, Goncalo [4 ]
Bomer, Moritz [4 ]
Atiri, Gabriel I. [2 ]
Onyeka, Joseph [3 ]
Maroya, Norbert G. [1 ]
Seal, Susan E. [4 ]
Kumar, P. Lava [1 ]
机构：
[1] IITA, Oyo Rd,PMB 5320, Ibadan, Nigeria
[2] Univ Ibadan, Dept Crop Protect & Environm Biol, Ibadan, Nigeria
[3] Natl Root Crops Res Inst, Umudike, Nigeria
[4] Univ Greenwich, Nat Resources Inst, Chatham ME4 4TB, Kent, England
来源：
ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 04期
基金：
比尔及梅琳达.盖茨基金会;
关键词：
RECOMBINASE POLYMERASE AMPLIFICATION; PCR; ASSAY;
D O I：
10.1007/s00705-018-3706-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity.
引用
收藏
页码：1057 / 1061
页数：5
相关论文
共 50 条
  • [1] Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the Rapid and Sensitive Detection of Yam mosaic virus
    Festus, Ruth O.
    Seal, Susan E.
    Prempeh, Ruth
    Quain, Marian D.
    Silva, Goncalo
    VIRUSES-BASEL, 2023, 15 (07):
  • [2] Development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Sugarcane mosaic virus and Sorghum mosaic virus in sugarcane
    Keizerweerd, Amber T.
    Chandra, Amaresh
    Grisham, Michael P.
    JOURNAL OF VIROLOGICAL METHODS, 2015, 212 : 23 - 29
  • [3] Rapid Visual Detection Method for Barley Yellow Mosaic Virus Using Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)
    Chen, Zhiwei
    Mao, Shuihua
    Zhang, Wan
    Fan, Xiaorui
    Wu, Wenjing
    Liu, Chenghong
    Zhao, Kai
    Lu, Ruiju
    PLANT DISEASE, 2021, 105 (09) : 2658 - 2663
  • [4] Rapid Detection of Tembusu Virus by Reverse-Transcription, Loop-mediated Isothermal Amplification (RT-LAMP)
    Tang, Y.
    Diao, Y.
    Yu, C.
    Gao, X.
    Chen, L.
    Zhang, D.
    TRANSBOUNDARY AND EMERGING DISEASES, 2012, 59 (03) : 208 - 213
  • [5] Use of Reverse Transcription Loop-Mediated Isothermal Amplification for the Detection of Zucchini yellow mosaic virus
    Kuan, Cheng-Ping
    Deng, Ting-Chin
    Huang, Hung-Chang
    Chi, Hsiang-Hui
    Lu, Yi-Lin
    JOURNAL OF PHYTOPATHOLOGY, 2014, 162 (04) : 238 - 244
  • [6] A Bioengineered Positive Control for Rapid Detection of the Ebola Virus by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)
    Lam, Patricia
    Keri, Ruth A.
    Steinmetz, Nicole F.
    ACS BIOMATERIALS SCIENCE & ENGINEERING, 2017, 3 (03): : 452 - 459
  • [7] Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
    Phusantisampan, Theerawut
    Rawiwan, Pattarasuda
    Roy, Sri Rajiv Kumar
    Sriariyanun, Malinee
    Surachetpong, Win
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2020, (159):
  • [8] Analysis of a Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) for yellow fever diagnostic
    Nunes, Marcio R. T.
    Vianez, Joau Lidio, Jr.
    Nunes, Keley N. B.
    da Silva, Sandro Patroca
    Lima, Clayton P. S.
    Guzman, Hilda
    Martins, Livia C.
    Carvalho, Valeria L.
    Tesh, Robert B.
    Vasconcelos, Pedro F. C.
    JOURNAL OF VIROLOGICAL METHODS, 2015, 226 : 40 - 51
  • [9] Development and evaluation of rapid detection of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)
    Yin, Shuanghui
    Shang, Youjun
    Zhou, Guangqing
    Tian, Hong
    Liu, Yanhong
    Cai, Xuepeng
    Liu, Xiangtao
    JOURNAL OF BIOTECHNOLOGY, 2010, 146 (04) : 147 - 150
  • [10] Fast detection of Southern tomato virus by one-step transcription loop-mediated isothermal amplification (RT-LAMP)
    Elvira-Gonzalez, L.
    Puchades, A. V.
    Carpino, C.
    Alfaro-Fernandez, A.
    Font-San-Ambrosio, M. I.
    Rubio, L.
    Galipienso, L.
    JOURNAL OF VIROLOGICAL METHODS, 2017, 241 : 11 - 14
12345