Chiral ligand-exchange chromatography for separation of three stereoisomers of octahydroindole-2-carboxylic acid

被引：11

作者：

Sun, SY

Jia, YZ

Zeng, N

Li, FM ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China

[2] Shanghai Inst Pharmaceut Ind, Shanghai 200437, Peoples R China

来源：

CHROMATOGRAPHIA | 2006年 / 63卷 / 7-8期

关键词：

column liquid chromatography; diastereo-enantio separation; chiral mobile phase; ligand-exchange; octohydroindole-2-carboxylic acid;

D O I：

10.1365/s10337-006-0752-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel HPLC method is described for separation of the three stereoisomers of octahydroinclole-2-carboxylic acid (Oic), an intermediate in the synthesis of perindopril. The chiral mobile phase contained the complex of Cu(II) with the optically active selector L-phenylalaninamide (L-PheA), and an ion-pair reagent, sodium 1-octanesulfonate. The effects of the concentrations of the Cu(II)-L-PheA complex and the ion-pair reagent, mobile phase pH, ionic strength, acetonitrile content, and column temperature were studied. Satisfactory resolution was achieved for three stereoisomers, RRR-, SSS-, and SRR-Oic.

引用

页码：331 / 335

页数：5

共 18 条

[1] COPPER(II) COMPLEXES OF DIAMINO-DIAMIDO-TYPE LIGANDS AS CHIRAL ELUENTS IN THE ENANTIOMERIC SEPARATION OF D,L-DANSYLAMINO ACIDS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].