Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity

被引:336
作者
Sasagawa, Yohei [1 ,7 ]
Nikaido, Itoshi [1 ,7 ]
Hayashi, Tetsutaro [2 ]
Danno, Hiroki [3 ]
Uno, Kenichiro D. [1 ]
Imai, Takeshi [4 ,5 ]
Ueda, Hiroki R. [1 ,3 ,6 ]
机构
[1] RIKEN Ctr Dev Biol, Funct Genom Unit, Chuo Ku, Kobe, Hyogo 6500047, Japan
[2] RIKEN Ctr Dev Biol, Genome Resource & Anal Unit, Chuo Ku, Kobe, Hyogo 6500047, Japan
[3] RIKEN Ctr Dev Biol, Lab Syst Biol, Chuo Ku, Kobe, Hyogo 6500047, Japan
[4] RIKEN Ctr Dev Biol, Lab Sensory Circuit Format, Chuo Ku, Kobe, Hyogo 6500047, Japan
[5] JST, PRESTO, Chuo Ku, Kobe, Hyogo 6500047, Japan
[6] RIKEN, Quantitat Biol Ctr, Lab Synthet Biol, Chuo Ku, Kobe, Hyogo 6500047, Japan
[7] RIKEN, Adv Ctr Comp & Commun, Bioinformat Res Unit, Wako, Saitama 3510198, Japan
关键词
Single cell; RNA-seq; Transcriptome; Sequencing; Bioinformatics; Cellular heterogeneity; Cell biology; OLIGONUCLEOTIDE MICROARRAY ANALYSIS; ES CELLS; CDNA AMPLIFICATION; STEM-CELLS; NOISE; TRANSCRIPTOME; LANDSCAPE;
D O I
10.1186/gb-2013-14-4-r31
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Development of a highly reproducible and sensitive single-cell RNA sequencing (RNA-seq) method would facilitate the understanding of the biological roles and underlying mechanisms of non-genetic cellular heterogeneity. In this study, we report a novel single-cell RNA-seq method called Quartz-Seq that has a simpler protocol and higher reproducibility and sensitivity than existing methods. We show that single-cell Quartz-Seq can quantitatively detect various kinds of non-genetic cellular heterogeneity, and can detect different cell types and different cell-cycle phases of a single cell type. Moreover, this method can comprehensively reveal gene-expression heterogeneity between single cells of the same cell type in the same cell-cycle phase.
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页数:17
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