Comparative pharmacokinetics study of three anthraquinones in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang by high performance liquid chromatography-mass spectrometry

被引：27

作者：

Li, Huan ^{[1
,2
,3
]}

Guo, Hui ^{[1
,2
]}

Wu, Li ^{[1
,2
]}

Zhang, Yongxin ^{[1
,2
]}

Chen, Juan ^{[1
]}

Liu, Xiao ^{[1
,2
]}

Cai, Hao ^{[1
,2
]}

Zhang, Kewei ^{[1
]}

Cai, Baochang ^{[1
,2
,3
]}

机构：

[1] Nanjing Univ Chinese Med, Coll Pharm, Nanjing 210046, Jiangsu, Peoples R China

[2] Nanjing Univ Chinese Med, Ctr Engn, State Minist Educ Standardizat Chinese Med Proc, Nanjing 210029, Jiangsu, Peoples R China

[3] Nanjing Haichang Chinese Med Grp Corp, Nanjing 210061, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2013年 / 76卷

基金：

中国国家自然科学基金;

关键词：

Pharmacokinetics; Rhein; Aloe-emodin; Emodin; CANCER-CELLS; ALOE-EMODIN;

D O I：

10.1016/j.jpba.2012.12.004

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A specific and sensitive high performance liquid chromatography-mass spectrometric (HPLC-MS) method has been developed and validated for simultaneous determination of three anthraquinones of rhein, aloe-emodin and emodin in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang. The analytes were separated on a Kromaisl (R) C-18 column within a total running time of 12 min with a mobile phase of methanol:ammonium acetate (3 mM) (75:25, v/v). The calibration curves for all the anthraquinones showed good linearity in the measured range with correlation coefficient (r) higher than 0.9978. The precision, accuracy, recovery and stability were deemed acceptable. The method was successfully applied to the comparative pharmacokinetics study of the anthraquinones in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：215 / 218

页数：4

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