Engineering Clostridium acetobutylicum for alcohol production

被引:34
作者
Hou, Xiaohu [1 ,2 ]
Peng, Wanfeng [2 ]
Xiong, Lian [2 ]
Huang, Chao [2 ]
Chen, Xuefang [2 ]
Chen, Xinde [2 ]
Zhang, Weiguo [1 ]
机构
[1] JiangNan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China
[2] Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
关键词
C; acetobutylicum; Alcohol production; adc gene inactivation; gshAB; Expression cassette (EC); Sol operon; FERMENTATIVE BUTANOL PRODUCTION; DOWN-REGULATION; ATCC; 824; TRANSCRIPTIONAL REGULATION; MOLECULAR CHARACTERIZATION; GENE; DISRUPTION; EXPRESSION; BUTYRATE; THIOLASE;
D O I
10.1016/j.jbiotec.2013.04.013
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
While Clostridium acetobutylicum has been used for large-scale butanol production (ABE fermentation), its by-product acetone cannot be used as a biofuel. In this study, C. acetobutylicum was engineered for alcohol titers (butanol plus ethanol). The adc gene was inactivated to eliminate acetone production, and glutathione biosynthetic capability was introduced into C. acetobutylicum to improve the strain's robustness by expressing Escherichia coli's gshAB genes in the adc locus. Acetone production was reduced from 2.64 +/- 0.22 g/L to 0.15 +/- 0.08 g/L in the engineered strain 824adc::gsh, whereas butanol production was increased from 5.17 +/- 0.26 g/L to 8.27 +/- 0.27 g/L. To further improve the alcohol titers, the metabolic flux in the alcohol biosynthesis pathways was enhanced. Overlapping PCR was used to generate expression cassette EC, which expresses the hbd, thl, crt, and bcd genes, and the Sol operon was amplified to express the adhE and ctfAB genes. Butanol and alcohol production reached 14.86 +/- 0.26 g/L and 18.11 +/- 0.66 g/L, respectively, in 824adc:: gsh Sol-EC. Furthermore, the butanol and alcohol yields were 0.336 g/g and 0.409 g/g, respectively, in 824adc:: gsh Sol-EC. This study provided a combined strategy for enhancing alcohol production in C. acetobutylicum. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:25 / 33
页数:9
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