Application of lectin microarray to crude samples: Differential glycan profiling of Lec mutants

被引:52
作者
Ebe, Y
Kuno, A
Uchiyama, N
Koseki-Kuno, S
Yamada, M
Sato, T
Narimatsu, H
Hirabayashi, J
机构
[1] Moritex Corp, Yokohama Tech Ctr, Nano Bio Sci Res Lab, Aoba Ku, Yokohama, Kanagawa 2250012, Japan
[2] Natl Inst Adv Ind Sci & Technol, RCG, Tsukuba, Ibaraki 3058568, Japan
关键词
evanescent; flow cytometry; glycan profiling; lectin; Lec mutant; microarray;
D O I
10.1093/jb/mvj070
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lee mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.
引用
收藏
页码:323 / 327
页数:5
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