Application of lectin microarray to crude samples: Differential glycan profiling of Lec mutants

被引：53

作者：

Ebe, Y

Kuno, A

Uchiyama, N

Koseki-Kuno, S

Yamada, M

Sato, T

Narimatsu, H

Hirabayashi, J

机构：

[1] Moritex Corp, Yokohama Tech Ctr, Nano Bio Sci Res Lab, Aoba Ku, Yokohama, Kanagawa 2250012, Japan

[2] Natl Inst Adv Ind Sci & Technol, RCG, Tsukuba, Ibaraki 3058568, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 2006年 / 139卷 / 03期

关键词：

evanescent; flow cytometry; glycan profiling; lectin; Lec mutant; microarray;

D O I：

10.1093/jb/mvj070

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lee mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.

引用

收藏

页码：323 / 327

页数：5

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