Further genetic characterization of the two Trypanosoma cruzi Berenice strains (Be-62 and Be-78) isolated from the first human case of Chagas disease (Chagas, 1909)

被引:9
作者
Cruz, RE
Macedo, AM
Barnabé, C
Freitas, JM
Chiari, E
Veloso, VM
Carneiro, CM
Bahia, MT
Tafuri, WL
Lana, M
机构
[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-30000 Belo Horizonte, MG, Brazil
[2] CNRS, IRD, UMR 9926, Genet Malad Infect, F-34394 Montpellier 05, France
[3] UFMG, Dept Parasitol, Belo Horizonte, MG, Brazil
[4] Univ Fed Ouro Preto, Escola Farm, Dept Anal Clin, BR-35400000 Ouro Preto, MG, Brazil
关键词
Trypanosoma cruzi; Berenice strains; isoenzyme; RAPD; microssatellites;
D O I
10.1016/j.actatropica.2005.03.011
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
We describe here an extension of a previous genetic characterization of Trypanosoma cruzi strains (Be-62 and Be-78) isolated from the patient Berenice, the first human case of Chagas disease [Chagas, C., 1909. Nova Tripanomiase humana. Estudos sobre morfologia e o ciclo evolutivo do Schizotrypanum cruzi, n. gen., n. sp., agente etiolojico da nova entidade morbida do homem. Mem. Inst. Oswaldo Cruz 1, 159-218]. We wanted to verify the composition of T. Cruzi Populations originated from these two isolates. In the present work, 22 enzymatic loci (MLEE), nine RAPID primers and 7 microsatellite loci were analyzed. Clones from both strains were also characterized to verify whether these strains are mono or polyclonal. Be-62 and Be-78 strains were different in 3 Out of 22 enzymatic systems, in 3 Out of 9 RAPD primers tested and in all microsatellite loci investigated. However, our data suggests that both strains are phylogenetically closely related, belonging to genetic group 32 front Tibayrenc and Ayala [Tibayrenc, M., Ayala, F J., 1988. Isoenzinne variability in Trypanosoma cruzi, the agent of Chagas' disease: genetical, taxonomical, and epidemiological significance. Evolution 42, 277-292], equivalent to zymodeme 2 and T. cruzi 11 major lineage which, in Brazil, comprises parasites from the domestic cycle of the disease. Microsatellite analyses showed differences between the parental strains but suggested that both populations are monoclonal since each strain and their respective clones showed the same amplification products. (c) 2005 Published by Elsevier B.V.
引用
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页码:239 / 246
页数:8
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