Abrupt Emergence of a Single Dominant Multidrug-Resistant Strain of Escherichia coli

被引：213

作者：

Johnson, James R. ^{[1
,2
]}

Tchesnokova, Veronika ^{[3
]}

Johnston, Brian ^{[1
,2
]}

Clabots, Connie ^{[1
,2
]}

Roberts, Pacita L. ^{[4
]}

Billig, Mariya ^{[3
]}

Riddell, Kim ^{[5
]}

Rogers, Peggy ^{[5
]}

Qin, Xuan ^{[6
]}

Butler-Wu, Susan ^{[7
]}

Price, Lance B. ^{[8
]}

Aziz, Maliha ^{[8
]}

Nicolas-Chanoine, Marie-Helene ^{[9
]}

DebRoy, Chitrita ^{[10
]}

Robicsek, Ari ^{[11
]}

Hansen, Glen ^{[12
]}

Urban, Carl ^{[13
,14
]}

Platell, Joanne ^{[15
]}

Trott, Darren J. ^{[16
]}

Zhanel, George ^{[17
]}

Weissman, Scott J. ^{[18
]}

Cookson, Brad T. ^{[3
,7
]}

Fang, Ferric C. ^{[3
,7
]}

Limaye, Ajit P. ^{[4
]}

Scholes, Delia ^{[19
]}

Chattopadhyay, Sujay ^{[3
]}

Hooper, David C. ^{[20
,21
]}

Sokurenko, Evgeni V. ^{[3
]}

机构：

[1] VA Med Ctr, Minneapolis, MN 55417 USA

[2] Univ Minnesota, Minneapolis, MN USA

[3] Univ Washington, Sch Med, Dept Microbiol, Seattle, WA 98195 USA

[4] Univ Washington, Sch Med, Dept Med, Seattle, WA 98195 USA

[5] Grp Hlth Cooperat Puget Sound, Grp Hlth Clin Lab, Seattle, WA USA

[6] Seattle Childrens Hosp, Dept Lab Med, Seattle, WA USA

[7] Univ Washington, Sch Med, Dept Lab Med, Seattle, WA 98195 USA

[8] Translat Genom Res Inst TGen, Flagstaff, AZ USA

[9] Hop Beaujon, Clichy, France

[10] Penn State Univ, University Pk, PA 16802 USA

[11] NorthShore Univ HealthSyst, Evanston, IL USA

[12] Hennepin Cty Med Ctr, Mineapolis, MN USA

[13] New York Hosp Queens, Flushing, NY USA

[14] NYU, Sch Med, New York, NY USA

[15] Biosecur Queensland, Anim Dis Surveillance Lab, Toowoomba, Qld, Australia

[16] Univ Adelaide, Roseworthy, Australia

[17] Univ Manitoba, Winnipeg, MB, Canada

[18] Seattle Childrens Res Inst, Seattle, WA USA

[19] Grp Hlth Cooperat Puget Sound, Grp Hlth Res Inst, Seattle, WA USA

[20] Massachusetts Gen Hosp, Dept Med, Boston, MA 02114 USA

[21] Massachusetts Gen Hosp, Dept Infect Dis, Boston, MA 02114 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2013年 / 207卷 / 06期

关键词：

Escherichia coli infections; antimicrobial resistance; extended-spectrum beta-lactamase; CTX-M-15; fluoroquinolone resistance; multidrug resistance; sequence type ST131; multilocus sequence typing; molecular epidemiology; FimH; SEQUENCE TYPE ST131; EXTRAINTESTINAL INFECTIONS; CIPROFLOXACIN-RESISTANT; CLONAL GROUPS; MECHANISMS; ANIMALS;

D O I：

10.1093/infdis/jis933

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. Fluoroquinolone-resistant Escherichia coli are increasingly prevalent. Their clonal origins-potentially critical for control efforts-remain undefined. Methods. Antimicrobial resistance profiles and fine clonal structure were determined for 236 diverse-source historical (1967-2009) E. coli isolates representing sequence type ST131 and 853 recent (2010-2011) consecutive E. coli isolates from 5 clinical laboratories in Seattle, Washington, and Minneapolis, Minnesota. Clonal structure was resolved based on fimH sequence (fimbrial adhesin gene: H subclone assignments), multilocus sequence typing, gyrA and parC sequence (fluoroquinolone resistance-determining loci), and pulsed-field gel electrophoresis. Results. Of the recent fluoroquinolone-resistant clinical isolates, 52% represented a single ST131 subclonal lineage, H30, which expanded abruptly after 2000. This subclone had a unique and conserved gyrA/parC allele combination, supporting its tight clonality. Unlike other ST131 subclones, H30 was significantly associated with fluoroquinolone resistance and was the most prevalent subclone among current E. coli clinical isolates, overall (10.4%) and within every resistance category (11%-52%). Conclusions. Most current fluoroquinolone-resistant E. coli clinical isolates, and the largest share of multidrug-resistant isolates, represent a highly clonal subgroup that likely originated from a single rapidly expanded and disseminated ST131 strain. Focused attention to this strain will be required to control the fluoroquinolone and multidrug-resistant E. coli epidemic.

引用

页码：919 / 928

页数：10

共 26 条

[21] Comparison of Asymptomatic Bacteriuria Escherichia coli Isolates from Healthy Individuals versus Those from Hospital Patients Shows that Long-Term Bladder Colonization Selects for Attenuated Virulence Phenotypes [J].

Salvador, Ellaine ;

Wagenlehner, Florian ;

Koehler, Christian-Daniel ;

Mellmann, Alexander ;

Hacker, Joerg ;

Svanborg, Catharina ;

Dobrindt, Ulrich .

INFECTION AND IMMUNITY, 2012, 80 (02) :668-678

[22] Adaptation to the fitness costs of antibiotic resistance in Escherichia coli [J].

Schrag, SJ ;

Perrot, V ;

Levin, BR .

PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1997, 264 (1386) :1287-1291

[23]

Swofford DL, 1998, PAUP PHYLOGENETIC AN

[24]

Tufan ZK, 2011, UROL J, V8, P69

[25] High-Resolution Two-Locus Clonal Typing of Extraintestinal Pathogenic Escherichia coli [J].

Weissman, Scott J. ;

Johnson, James R. ;

Tchesnokova, Veronika ;

Billig, Mariya ;

Dykhuizen, Daniel ;

Riddell, Kim ;

Rogers, Peggy ;

Qin, Xuan ;

Butler-Wu, Susan ;

Cookson, Brad T. ;

Fang, Ferric C. ;

Scholes, Delia ;

Chattopadhyay, Sujay ;

Sokurenko, Evgeni .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (05) :1353-1360

[26] Clonal analysis reveals high rate of structural mutations in fimbrial adhesins of extraintestinal pathogenic Escherichia coli [J].

Weissman, SJ ;

Chattopadhyay, S ;

Aprikian, P ;

Obata-Yasuoka, M ;

Yarova-Yarovaya, Y ;

Stapleton, A ;

Ba-Thein, W ;

Dykhuizen, D ;

Johnson, JR ;

Sokurenko, EV .

MOLECULAR MICROBIOLOGY, 2006, 59 (03) :975-988

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