TALEN-mediated precise genome modification by homologous recombination in zebrafish

被引：228

作者：

Zu, Yao ^{[1
,2
]}

Tong, Xiangjun ^{[1
]}

Wang, Zhanxiang ^{[1
]}

Liu, Da ^{[1
]}

Pan, Ruochuan ^{[1
]}

Li, Zhe ^{[1
]}

Hu, Yingying ^{[1
]}

Luo, Zhou ^{[1
]}

Huang, Peng ^{[1
]}

Wu, Qian ^{[1
]}

Zhu, Zuoyan ^{[1
]}

Zhang, Bo ^{[1
]}

Lin, Shuo ^{[2
]}

机构：

[1] Peking Univ, Coll Life Sci, Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China

[2] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90024 USA

来源：

NATURE METHODS | 2013年 / 10卷 / 04期

基金：

中国国家自然科学基金; 美国国家卫生研究院;

关键词：

ZINC-FINGER NUCLEASES; TARGETED GENE DISRUPTION; DESIGN; CELLS; MICE;

D O I：

10.1038/NMETH.2374

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report gene targeting via homologous recombination in zebrafish. We co-injected fertilized eggs with transcription activator-like effector nuclease mRNAs and a donor vector with long homologous arms targeting the tyrosine hydroxylase (th) locus, and we observed effective gene modification that was transmitted through the germ line. We also successfully targeted two additional genes. Homologous recombination in zebrafish with a dsDNA donor expands the utility of this model organism.

引用

页码：329 / +

页数：4

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