Immunolocalisation of sodium/proton exchanger-like proteins in the gills of elasmobranchs

被引:40
作者
Edwards, SL
Donald, JA
Toop, T [1 ]
Donowitz, M
Tse, CM
机构
[1] Deakin Univ, Sch Biol & Chem Sci, Geelong, Vic 3217, Australia
[2] Johns Hopkins Univ, Sch Med, Dept Med & Physiol, Div Gastroenterol, Baltimore, MD USA
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY | 2002年 / 131卷 / 02期
关键词
acid/base regulation; elasmobranch; gills; sodium/proton exchanger; Na+/H+ exchanger (NHE); mitochondria-rich cells;
D O I
10.1016/S1095-6433(01)00449-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Na+/H+ exchangers are integral membrane proteins that exchange Na+ and H+ across cell membranes. The Na+/H+ exchangers 2 and 3 are epithelial isoforms in mammals and contribute to acid-base homeostasis. The gills of fishes, including elasmobranchs, are also associated with acid/base balance, and are probably the primary acid/base regulatory organ. This study examines the presence of Na+/H+ exchangers 2 and 3 using immunohistochemistry and immunoblotting in the gills of four species of elasmobranchs, the banjo ray (Trygonorrhina fasciata), southern eagle ray (Myliobatis australis), the gummy shark (Mustelus antarcticus) and the Australian angel shark (Squatina australis) using heterologous antibodies. Na+/H+ exchanger 2-like immunoreactivity was observed in the gills of the banjo ray, eagle ray and angel shark. In the banjo and eagle rays, this Na+/H+ exchanger 2-like immunoreactivity co-localised with immunoreactivity to Na+/K+-ATPase, a marker for the mitochondrial-rich cells of fishes. Na+/H+ exchanger 3-like immunoreactivity was only observed in the gills of the angel and gummy sharks, some Na+/H+ exchanger 3-like cells also showed Na+/K+-ATPase immunoreactivity. However, immunoblotting of banjo and eagle ray gill membranes demonstrated Na+/H+ exchanger 3-like immunoreactivity, which was not consistent with the immunohistochemical results. These data demonstrate the presence of epithelial Na+/H+ exchangers 2 and 3 in the gills of elasmobranchs and a link with acid/base regulation is suggested. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:257 / 265
页数:9
相关论文
共 39 条
[1]   Molecular cloning and functional expression of a rat Na+/H+ exchanger (NHE5) highly expressed in brain [J].
Attaphitaya, S ;
Park, K ;
Melvin, JE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (07) :4383-4388
[2]   Molecular cloning, genomic organization, and functional expression of Na+/H+ exchanger isoform 5 (NHE5) from human brain [J].
Baird, NR ;
Orlowski, J ;
Szabó, EZ ;
Zaun, HC ;
Schultheis, PJ ;
Menon, AG ;
Shull, GE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (07) :4377-4382
[3]   CLONING AND EXPRESSION OF A CAMP-ACTIVATED NA+/H+ EXCHANGER - EVIDENCE THAT THE CYTOPLASMIC DOMAIN MEDIATES HORMONAL-REGULATION [J].
BORGESE, F ;
SARDET, C ;
CAPPADORO, M ;
POUYSSEGUR, J ;
MOTAIS, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (15) :6765-6769
[4]  
Chow CW, 1999, CLIN INVEST MED, V22, P195
[5]  
Claiborne JB, 1997, J EXP ZOOL, V279, P509, DOI 10.1002/(SICI)1097-010X(19971201)279:5<509::AID-JEZ15>3.0.CO
[6]  
2-2
[7]   ACID-BASE-BALANCE AND ION TRANSFERS IN THE SPINY DOGFISH (SQUALUS-ACANTHIAS) DURING HYPERCAPNIA - A ROLE FOR AMMONIA EXCRETION [J].
CLAIBORNE, JB ;
EVANS, DH .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1992, 261 (01) :9-17
[8]  
CLAIBORNE JB, 1998, PHYSL FISHES, P179
[9]   HISTOCHEMICAL-LOCALIZATION OF NA+-K+ ATPASE AND CARBONIC-ANHYDRASE ACTIVITY IN GILLS OF 17 FISH SPECIES [J].
CONLEY, DM ;
MALLATT, J .
CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE, 1988, 66 (11) :2398-2405
[10]   Immunolocalisation of NHE3-like immunoreactivity in the gills of the rainbow trout (Oncorhynchus mykiss) and the blue-throated wrasse (Pseudolabrus tetrious) [J].
Edwards, SL ;
Tse, CM ;
Toop, T .
JOURNAL OF ANATOMY, 1999, 195 :465-469