Structural elucidation of a polysaccharide from Chrysanthemum morifolium flowers with anti-angiogenic activity

被引：41

作者：

Zheng, Changping ^{[1
,2
]}

Dong, Qun ^{[1
]}

Du, Zhenyun ^{[1
]}

Wang, Peipei ^{[1
]}

Ding, Kan ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Mat Med, Glycobiol & Glycochem Lab, Shanghai 201203, Peoples R China

[2] Nanchang Univ, Coll Pharm, Nanchang 330000, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 79卷

基金：

中国国家自然科学基金;

关键词：

Chrysanthemum morifolium; Polysaccharide; Anti-angiogenesis; PLATYCODON-GRANDIFLORUM; ANTIANGIOGENIC ACTIVITY; GRIFOLA-FRONDOSA; ARABINOGALACTAN; OLIGOSACCHARIDES; BIOACTIVITY; XYLOGLUCAN; INHIBITION; FEATURES; EXTRACT;

D O I：

10.1016/j.ijbiomac.2015.04.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The flowers of Chrysanthemum morifolium were extracted with 5% sodium hydroxide, yielding a neutral polysaccharide (JHB0S2) with a molecular weight of 16 kDa by anion-exchange chromatography on a DEAE-cellulose column and gel permeation chromatography on a Sephacryl S-200 HR column. JHB0S2 was found to contain glucose, xylose, arabinose and galactose in molar ratio of 2.9:2.3:1.0:1.2. Through the linkage analysis, partial acid hydrolysis and NMR spectra and so forth, indicated that its back-bone consisted of 1,4-linked beta-Glcp, nearly 50% of which was substituted at O-6 by 1,2-linked alpha-Xylp terminated with Galp or Xylp. Furthermore, some of 1,2-linked alpha-Xylp was substituted at O-4 by alpha-Araf-(1 -> 5)-alpha-Araf-(1 -> 6)-beta-Galp. The biological test showed that JHB0S2 could inhibit the tube formation of HMEC-1 cells. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：674 / 680

页数：7

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