Deletion of RAR carboxyl terminus reveals promoter- and receptor-specific AF-1 effects

被引:6
作者
Aneskievich, BJ [1 ]
机构
[1] Univ Connecticut, Sch Pharm, Dept Pharmaceut Sci, Storrs, CT 06269 USA
关键词
retinoic acid receptor; activating function 1; keratinocyte; differentiation; epidermis;
D O I
10.1006/bbrc.2001.6081
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Retinoic acid receptors (RARs) are transcription factors with both amino-terminal ligand-independent and carboxyl-terminal ligand-dependent activation functions (AF-1 and AF-2, respectively). RAR-dependent gene activation in keratinocytes was investigated via expression of varied RARalpha and RARgamma carboxyl terminal truncation mutants lacking the AF-2 domain. Overexpression of the AF-1 domain of RARalpha or RARgamma was sufficient to decrease transcriptional activation of retinoid-dependent genes in keratinocytes. Conversely, expression of the same constructs was associated with an increase in expression of endogenous and synthetic reporter genes otherwise negatively regulated by RARs. These effects on transcription driven by some but not all retinoid-sensitive promoters tested could be alleviated by mutation of a serine phosphorylation site in the A/B domain. These results further support the promoter-specificity previously attributed to the RAR AF-1 region and functionally define a particular amino acid residue likely to contribute to the regulation of RARs and other proteins in the transcription complex. (C) 2001 Elsevier Science.
引用
收藏
页码:950 / 956
页数:7
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