Gene silencing and large-scale domain structure of the E. coli genome

被引:14
作者
Zarei, Mina [1 ,2 ,3 ]
Sclavi, Bianca [4 ]
Lagomarsino, Marco Cosentino [1 ,2 ,5 ]
机构
[1] CNRS, Genophys Genom Phys Grp, UMR Microorganism Genom 7238, Paris, France
[2] Univ Paris 06, F-75006 Paris, France
[3] Univ Milan, Dipartimento Fis, I-20133 Milan, Italy
[4] Ecole Normale Super, CNRS, LBPA, UMR 8113, F-94235 Cachan, France
[5] Univ Turin, Dipartimento Fis, Turin, Italy
关键词
HORIZONTALLY TRANSFERRED GENES; NUCLEOID-ASSOCIATED PROTEINS; ESCHERICHIA-COLI; H-NS; CHROMOSOME-REPLICATION; BACTERIAL CHROMATIN; CURVED DNA; ORGANIZATION; EXPRESSION; TRANSCRIPTION;
D O I
10.1039/c3mb25364c
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The H-NS chromosome-organizing protein in E. coli can stabilize genomic DNA loops, and form oligomeric structures connected to repression of gene expression. Motivated by the link between chromosome organization, protein binding and gene expression, we analyzed publicly available genomic data sets of various origins, from genome-wide protein binding profiles to evolutionary information, exploring the connections between chromosomal organization, gene-silencing, pseudo-gene localization and horizontal gene transfer. We report the existence of transcriptionally silent contiguous areas corresponding to large regions of H-NS protein binding along the genome, their position indicates a possible relationship with the known large-scale features of chromosome organization.
引用
收藏
页码:758 / 767
页数:10
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