A Quantitative Relaxometric Version of the ELISA Test for the Measurement of Cell Surface Biomarkers

被引：48

作者：

Alberti, Diego ^{[1
,2
]}

van't Erve, Mark ^{[1
,2
]}

Stefania, Rachele ^{[1
,2
]}

Ruggiero, Maria Rosaria ^{[1
,2
]}

Tapparo, Marta ^{[1
,2
]}

Crich, Simonetta Geninatti ^{[1
,2
]}

Aime, Silvio ^{[1
,2
]}

机构：

[1] Univ Turin, Dept Mol Biotechnol, Turin, Italy

[2] Univ Turin, Hlth Sci Inst, Turin, Italy

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 13期

关键词：

analytical methods; NMR spectroscopy; paramagnetic liposomes; personalized medicine; relaxometry; FOLATE-RECEPTOR EXPRESSION; PARAMAGNETIC LIPOSOMES; CONTRAST AGENTS; CANCER; DELIVERY; MRI; A(2);

D O I：

10.1002/anie.201310959

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Quantitative measurement of marker expression in diseased cells is still a topic of considerable interest and different methodologies are currently under intense scrutiny. This work aims at developing an invitro diagnostic method based on the release of paramagnetic species from relaxometrically silent liposomes operated by the action of a phospholipase A(2) (PLA(2)) previously targeted to the epitope of interest. The released paramagnetic species causes an increase of the longitudinal water proton relaxation rate proportional to the number of PLA(2) bound to the cell outer surface. The sensitivity of the herein proposed method, named R-ELISA, was attempted in the detection of folate receptor expression on human ovarian cancer cells by functionalizing PLA(2) with folic acid. Receptor/cell number of 8.3x10(5) has been measured on IGROV-1 cells. The R-ELISA assay can detect nanomolar cell suspension receptor concentrations and has been validated by well-established spectrofluorimetric procedures.

引用

页码：3488 / 3491

页数：4

共 27 条

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