Increased expression of tumor necrosis factor-α messenger RNA in the intestinal mucosa of inflammatory bowel disease, particularly in patients with disease in the inactive phase

被引：55

作者：

Akazawa, A ^{[1
]}

Sakaida, I ^{[1
]}

Higaki, S ^{[1
]}

Kubo, Y ^{[1
]}

Uchida, K ^{[1
]}

Okita, K ^{[1
]}

机构：

[1] Yamaguchi Univ, Sch Med, Dept Gastroenterol & Hepatol, Ube, Yamaguchi 7558505, Japan

来源：

JOURNAL OF GASTROENTEROLOGY | 2002年 / 37卷 / 05期

关键词：

tumor necrosis factor-alpha messenger RNA; inflammatory bowel disease; quantitative RT-PCR;

D O I：

10.1007/s005350200048

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background Tumor necrosis factor-alpha (TNF-alpha), may be involved in the pathogenesis of inflammatory bowel diseases (IBDs). The aim of this study was to evaluate the effect of TNF-alpha on the inflammatory activity of IBD. Methods. TNF-alpha mRNA expression in intestinal mucosal biopsy specimens from IBD patients [ulcerative colitis (UC), n = 54; and Crohn's disease (CD), n = 11] was analyzed using a competitive polymerase chain reaction. The degree of macrophage infiltration was analyzed by immunohistochemistry, using an antihuman CD68 antibody. Results. TNF-alpha mRNA expression was increased in UC patients, corresponding to the inflammatory activity. However, in CD, TNF-alpha mRNA expression was not correlated with the endoscopic findings. Conclusions. We clarified that TNF-alpha mRNA expression was responsible for the inflammatory activity in UC. However, TNF-alpha mRNA expression was not correlated with the mucosal injury in CD.

引用

页码：345 / 353

页数：9

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