Hydroxyurea promotes TET1 expression and induces apoptosis in osteosarcoma cells

被引：19

作者：

Teng, Songsong ^{[1
]}

Ma, Chunhui ^{[1
]}

Yu, Yinxian ^{[1
]}

Yi, Chengqing ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Sch Med, Dept Orthoped, Shanghai, Peoples R China

来源：

BIOSCIENCE REPORTS | 2019年 / 39卷

关键词：

CANCER; PROTEINS;

D O I：

10.1042/BSR20190456

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ten-eleven translocation (TET) proteins are abnormally expressed in various cancers. Osteosarcoma cells were treated with hydroxyurea to investigate the expression pattern of TET proteins in these cells. The expression of TET1 was increased in U2OS cells after treatment with hydroxyurea. In addition, hydroxyurea increased cell apoptosis and altered the cell cycle. TET proteins catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC); therefore, 5mC and 5hmC levels were evaluated. Increased 5hmC levels were observed after the hydroxyurea treatment. Experiments examining cell apoptosis and the cell cycle after knockdown and overexpression of TET1 were conducted to further investigate whether TET1 expression affected cell growth. The overexpression of TET1 increased cell apoptosis and inhibited cell growth. Taken together, TET1 expression regulated proliferation and apoptosis in U2OS cells, changes that were associated with 5hmC levels.

引用

页数：8

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