Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors

被引：66
作者：
Goparaju, Sravan Kumar [1 ]
Kohda, Kazuhisa [2 ]
Ibata, Keiji [2 ]
Soma, Atsumi [1 ]
Nakatake, Yukhi [1 ]
Akiyama, Tomohiko [1 ]
Wakabayashi, Shunichi [1 ]
Matsushita, Misako [1 ]
Sakota, Miki [1 ]
Kimura, Hiromi [1 ]
Yuzaki, Michisuke [2 ]
Ko, Shigeru B. H. [1 ]
Ko, Minoru S. H. [1 ]
机构：
[1] Keio Univ, Sch Med, Dept Syst Med, Shinjuku Ku, 35 Shinanomachi, Tokyo 1608582, Japan
[2] Keio Univ, Sch Med, Dept Physiol, Shinjuku Ku, 35 Shinanomachi, Tokyo 1608582, Japan
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
基金：
日本科学技术振兴机构;
关键词：
LOOP-HELIX FACTORS; HUMAN FIBROBLASTS; SPECIFICATION; GENERATION; INDUCTION; INHIBITION; DERIVATION; GENES; ESCS;
D O I：
10.1038/srep42367
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability of five transcription factors (TFs) toward this aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2. In contrast to previous methods that have shortcomings in their speed and efficiency, a cocktail of these TFs as synthetic mRNAs can differentiate hPSCs into neurons in 7 days, judged by calcium imaging and electrophysiology. They exhibit motor neuron phenotypes based on immunostaining. These results indicate the establishment of a novel method for rapid, efficient, and footprint-free differentiation of functional neurons from hPSCs.
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页数：12
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