Identification of a naturally occurring ligand for thymic positive selection

被引:167
作者
Hogquist, KA
Tomlinson, AJ
Kieper, WC
McGargill, MA
Hart, MC
Naylor, S
Jameson, SC
机构
[1] UNIV MINNESOTA,CTR IMMUNOL,MINNEAPOLIS,MN 55455
[2] MAYO CLIN & MAYO FDN,DEPT BIOCHEM & MOL BIOL,ROCHESTER,MN 55905
[3] MAYO CLIN & MAYO FDN,BIOMED MASS SPECTROMETRY FACIL,ROCHESTER,MN 55905
[4] WASHINGTON UNIV,DEPT CELL BIOL,ST LOUIS,MO 63110
关键词
D O I
10.1016/S1074-7613(00)80282-4
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In the thymus, positive and negative selection shape the T cell repertoire. it has previously been shown that positive selection, like negative selection, is the result of the interaction of the TCR with self-peptides bound to MHC. However, lime is known about the number or nature of the self-peptide ligands that mediate positive selection in vivo. We devised a novel assay with enhanced sensitivity for low affinity TCR ligands to identify self-peptides that may be biologically relevant. At least eight K-b-bound serf-peptides were detected by this assay using thymocytes bearing the OT-I TCR (specific for OVAp/K-b). The sequence of one of these peptides was determined using the recently developed technique of membrane preconcentration-capillary electrophoresis-tandem mass spectrometry. This peptide, CP alpha 1, has limited sequence similarity to OVAp, yet was found to induce positive selection of OT-I thymocytes in fetal thymic organ culture.
引用
收藏
页码:389 / 399
页数:11
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