Single Protein Molecule Detection by Glass Nanopores

被引：226

作者：

Li, Wenhong ^{[1
]}

Bell, Nicholas A. W. ^{[1
]}

Hernandez-Ainsa, Silvia ^{[1
]}

Thacker, Vivek V. ^{[1
]}

Thackray, Alana M. ^{[2
]}

Bujdoso, Raymond ^{[2
]}

Keyser, Ulrich F. ^{[1
]}

机构：

[1] Univ Cambridge, Cavendish Lab, Cambridge CB3 OHE, England

[2] Univ Cambridge, Dept Vet Med, Cambridge CB3 0ES, England

来源：

ACS NANO | 2013年 / 7卷 / 05期

基金：

英国工程与自然科学研究理事会;

关键词：

nanopores; nanocapillary; protein translocation; PrP; prions; single molecule detection; diagnosis; PRION PROTEIN; TRANSLOCATION; PEPTIDES;

D O I：

10.1021/nn4004567

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nanopores can be used to detect and analyze single molecules in solution. We have used glass nanopores made by laser-assisted capillary-pulling, as a high-throughput and low cost method, to detect a range of label-free proteins: lysozyme, avidin, IgG, beta-lactoglobulin, ovalbumin, bovine serum albumin (BSA), and beta-galactosidase in solution. Furthermore, we show for the first time solid state nanopore measurements of mammalian prion protein, which in its abnormal form is associated with transmissible spongiform encephalopathies. Our approach provides a basis for protein characterization and the study of protein conformational diseases by nanopore detection.

引用

页码：4129 / 4134

页数：6

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