Fast amperometric immunoplatform for ovomucoid traces determination in fresh and baked foods

被引：24

作者：

Benede, S. ^{[1
]}

Ruiz-Valdepenas Montiel, V. ^{[2
]}

Povedano, E. ^{[2
]}

Villalba, M. ^{[1
]}

Mata, L. ^{[3
]}

Galan-Malo, P. ^{[3
]}

Torrente-Rodriguez, R. M. ^{[2
]}

Vargas, E. ^{[2
]}

Reviejo, A. J. ^{[2
]}

Campuzano, S. ^{[2
]}

Pingarron, J. M. ^{[2
]}

机构：

[1] Univ Complutense Madrid, Fac Ciencias Quim, Dept Bioquim & Biol Mol 1, E-28040 Madrid, Spain

[2] Univ Complutense Madrid, Fac CC Quim, Dept Quim Analit, E-28040 Madrid, Spain

[3] ZEULAB SL, Bari 25, E-50194 Zaragoza, Spain

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 265卷

关键词：

Ovomucoid; Micromagnetic beads; Screen-printed electrode; Amperometric immunosensor; Unprocessed foods; Baked bread; ELECTROCHEMICAL IMMUNOSENSOR; MAGNETIC BEADS; PEANUT ALLERGEN; EGG PROTEINS; QUANTIFICATION; LACTOGLOBULIN; COMPONENTS; PLATFORM;

D O I：

10.1016/j.snb.2018.03.075

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes the first immunosensor reported so far for the highly sensitive determination of the egg white allergen, ovomucoid (OM). The approach involves the selective capture of sandwich immunocomplexes formed by capture antibody-target protein allergen-HRP-labeled detector antibody onto carboxylic acid-functionalized magnetic beads (HOOC-MBs). The resulting magnetic bioconjugates were captured on the surface of disposable screen-printed carbon electrodes (SPCE) to perform amperometric detection at -0.20V vs. the Ag pseudo-reference electrode in the presence of hydroquinone (HQ) and H2O2. The as prepared electrochemical immunoplatform showed a wide range of linearity (0.3-25 ng mL(-1)), a LOD of 0.1 ng mL(-1), which is much lower than those claimed for commercial ELISA kits, and a great selectivity against other white egg allergenic proteins. The developed immunosensor was successfully employed for the simple and accurate determination of trace amounts of OM in unprocessed (eggs and wheat flour) and baked (bread) food samples. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：421 / 428

页数：8

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