Cell image reconstruction using digital holography with an improved GS algorithm

被引:6
作者
Jiang, Yuhao [1 ,2 ]
Li, Hongzhong [3 ]
Pang, Yu [1 ]
Ling, Jiwei [1 ]
Wang, Hao [1 ]
Yang, Yuling [1 ]
Li, Xinyu [1 ]
Tian, Yin [1 ,4 ]
Wang, Xiuxin [1 ,5 ]
机构
[1] Chongqing Univ Posts & Telecommun, Inst Adv Sci, Chongqing, Peoples R China
[2] Cent Nervous Syst Drug Key Lab Sichuan Prov, Luzhou, Peoples R China
[3] Guangdong Commun Polytech, Sch Civil Engn, Guangzhou, Peoples R China
[4] Chongqing Inst Brain & Intelligence, Guangyang Bay Lab, Chongqing, Peoples R China
[5] Lanzhou Jiaotong Univ, Key Lab Optotechnol & Intelligent Control, Minist Educ, Lanzhou, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
computer hologram; cell image; GS algorithm; phase reconstruction; reconstruction; PHASE RETRIEVAL; MICROSCOPY; TOMOGRAPHY;
D O I
10.3389/fphys.2022.1040777
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Digital holography is an effective technology in image reconstruction as amplitude and phase information of cells can be acquired without any staining. In this paper, we propose a holographic technique with an improved Gerchberg-Saxton (GS) algorithm to reconstruct cell imaging based on phase reconstruction information. Comparative experiments are conducted on four specific models to investigate the effectiveness of the proposed method. The morphological parameters (such as shape, volume, and sphericity) of abnormal erythrocytes can be obtained by reconstructing cell hologram of urinary sediment. Notably, abnormal red blood cells can also be detected in mussy circumstances by the proposed method, owing to the significantly biophysical contrast (refractive index distribution and mass density) between two different cells. Therefore, this proposed method has a broad application prospect in cell image reconstruction and cell dynamic detection.
引用
收藏
页数:12
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