How Reversed-Phase Liquid Chromatography Works

被引：0

作者：

Schure, Mark R. ^{[1
]}

Rafferty, Jake L. ^{[2
,3
,4
]}

Zhang, Ling ^{[2
,3
]}

Siepmann, J. Ilja ^{[2
,3
,4
,5
]}

机构：

[1] Kroungold Analyt Inc, Superon & Theoret Separat Sci Lab, Blue Bell, PA USA

[2] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA

[3] Univ Minnesota, Chem Theory Ctr, Minneapolis, MN USA

[4] North Hennepin Community Coll, Dept Chem, Brooklyn Pk, MN USA

[5] Univ Minnesota, Dept Chem Engn & Mat Sci, Minneapolis, MN 55455 USA

来源：

LC GC NORTH AMERICA | 2013年 / 31卷 / 08期

基金：

美国国家科学基金会;

关键词：

MOLECULAR SIMULATION; RETENTION MECHANISM; STATIONARY-PHASE; CHAIN-LENGTH; WATER; ADSORPTION; CONFORMATION; COVERAGE;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The keys to understanding reversed-phase liquid chromatography (LC) are provided at the molecular mechanism level as determined by high accuracy molecular simulation. The essential features of C-18 stationary-phase chains in contact with methanol-water and acetonitrile-water mixtures are discussed in the context of bonded-chain geometry, spatial distribution of alkane and alcohol solutes, retention mechanism, and retention thermodynamics. This tutorial is intended to be applicable to a wide audience ranging from occasional users of liquid chromatography to separation scientists.

引用

页码：630 / 637

页数：8

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