Increased epidermal growth factor receptor (EGFR) expression in malignant mammary phyllodes tumors

被引:42
作者
Tse, Gary M. K. [1 ]
Lui, Philip C. W. [1 ]
Vong, Joaquim S. L. [1 ]
Lau, Kin-Mang [1 ]
Putti, Thomas C. [2 ]
Karim, Rooshdiya [3 ]
Scolyer, Richard A. [3 ]
Lee, C-Soon [3 ]
Yu, Alex M. C. [1 ]
Ng, David C. H. [1 ]
Tse, Agnes K. Y. [1 ]
Tan, Puay-Hoon [4 ]
机构
[1] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Anat & Cellular Pathol, Shatin, Hong Kong, Peoples R China
[2] Natl Univ Singapore, Dept Pathol, Singapore 117548, Singapore
[3] Univ Sydney, Dept Pathol, Fac Med, Sydney, NSW 2006, Australia
[4] Singapore Gen Hosp, Dept Pathol, Singapore 119074, Singapore
关键词
Breast; Epidermal growth factor receptor; Fluorescence in situ hybridization; Immunohistochemistry; Phyllodes tumors; PROTEIN EXPRESSION; C-KIT; TARGETED THERAPY; LUNG-CANCER; NECK-CANCER; BREAST; GENE; AMPLIFICATIONS; MUTATIONS; CD117;
D O I
10.1007/s10549-008-0030-5
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Mammary phyllodes tumors are uncommon stromal-epithelial neoplasms, and are divided into benign, borderline malignant and frankly malignant groups on the basis of their histological features. Accumulating evidence shows that epidermal growth factor receptor (EGFR) is involved in the pathogenesis and progression of many malignancies. This study investigated 453 phyllodes tumors (296 benign, 98 borderline, 59 malignant) for EGFR expression using immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) for gene amplification. The staining was correlated to tumor margin status, degree of malignancy, stromal cellularity, mitotic activity, nuclear pleomorphism and stromal overgrowth. Cases with strong positive IHC staining were selected for FISH. The overall positive rate for EGFR was 16.2% (48/296), 30.6% (30/98) and 56% (33/59) for benign, borderline malignant and frankly malignant phyllodes tumors, respectively. FISH demonstrated egfr gene amplification in 8% of immunohistochemically positive cases. The results of this study provide strong evidence that EGFR overexpression is involved in the pathogenesis of phyllodes tumors, although gene amplification may not be the major underlying mechanism for overexpression.
引用
收藏
页码:441 / 448
页数:8
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