Preferential recognition of peroxynitrite modified human DNA by circulating autoantibodies in cancer patients

被引：29

作者：

Habib, Safia ^{[1
]}

Ali, Moinuddin Asif ^{[1
]}

Ali, Rashid ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Fac Med, JN Med Coll, Dept Biochem, Aligarh 202002, Uttar Pradesh, India

来源：

CELLULAR IMMUNOLOGY | 2009年 / 254卷 / 02期

关键词：

Cancer autoantibodies; Reactive nitrogen species; Peroxynitrite modified DNA; DNA damage; NITRIC-OXIDE; REACTIVE OXYGEN; VARYING SIZE; DAMAGE; INFLAMMATION; BINDING; CHEMISTRY; ANTIGEN; NO;

D O I：

10.1016/j.cellimm.2008.08.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Peroxynitrite (ONOO-) has been vastly implicated in mutagenesis and cancer development. Present study probes the antigenicity of peroxynitrite damaged DNA (ONOO--DNA) in cancer patients. Purified human placental DNA was damaged by the synergistic action of sodium nitroprusside (SNP) and Pyrogallol for 3 h at 37 degrees C. Binding characteristics of cancer autoantibodies as well as experimentally induced anti-peroxynitrite-DNA (anti-ONOO--DNA) antibodies were assessed by ELISA and band shift assay. DNA modifications produced single strand breaks, decreased melting temperature (T-m), hyperchromicity in UV spectrum and decreased fluorescence intensity. The ONOO--DNA induced high titre antibodies in experimental animals. Cancer autoantibodies exhibited enhanced binding with the modified DNA as compared to the native form. Lymphocyte DNA from cancer patients showed appreciable recognition of anti ONCC--DNA IgG as compared to the DNA from healthy subjects. The peroxynitrite modified DNA presents unique epitopes which may be one of the factors for the autoantibody induction in cancer patients. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：117 / 123

页数：7

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