The expression of NOB1 in spiral ganglion cells of guinea pig

被引：8
作者：
Han, Yu [1 ]
Hong, Liu [2 ]
Zhong, Cuiping [1 ]
Xue, Tao [1 ]
He, Ya [1 ]
Chen, Jun [1 ]
Chunyu, Xiujie [1 ]
Qiao, Li [1 ]
Qiu, Jianhua [1 ]
机构：
[1] Fourth Mil Med Univ, Xijing Hosp, Dept Otolaryngol, Xian 710032, Shaanxi Prov, Peoples R China
[2] Fourth Mil Med Univ, Xijing Hosp, Div Digest Dis 1, Xian 710032, Shaanxi Prov, Peoples R China
来源：
INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY | 2009年 / 73卷 / 02期
关键词：
NOB1; Zinc ribbon domain; Immunohistochemistry; Guinea pigs; Spiral ganglion cells; GASTRIC-CANCER CELLS; MULTIDRUG-RESISTANCE; ZNRD1; GENE; PROLIFERATION; BCL-2;
D O I：
10.1016/j.ijporl.2008.10.028
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
Objective: NOB1 was a transcription-associated protein, consisting of one zinc ribbon domain. This study aimed to investigate the NOB1 expression in spiral ganglion cells of normal guinea pigs and deaf ones. Methods: Twelve guinea pigs were randomized equally into experimental group and control group. In experimental group, guinea pigs received a single intramuscular injection of gentamicin, while the control group was treated with physiological saline. Auditory brainstem responses (ABR) test was performed before and 10 days after injection, respectively. Guinea pigs of both groups were sacrificed and temporal bones were removed. The expression of NOB1 in the spiral ganglion was evaluated by immunohistochemistry. Results: NOB1 staining was found expressed in spiral ganglion cells from the basal turn to the apical turn of the cochlea. The expression of NOB1 was found significantly stronger in spiral ganglion cells of deaf guinea pigs as compared with that in normal ones. Conclusions: The NOB1 was presented in spiral ganglions cells of the guinea pig and might play a role in hearing transduction. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：315 / 319
页数：5
相关论文
共 12 条
[1]   Structural basis of transcription: An RNA polymerase II-TFIIB cocrystal at 4.5 angstroms [J].
Bushnell, DA ;
Westover, KD ;
Davis, RE ;
Kornberg, RD .
SCIENCE, 2004, 303 (5660) :983-988
[2]   Preparation and characterization of a novel monoclonal antibody specific to human NOB1 protein [J].
Han, Yu ;
Hong, Liu ;
Qiu, Jianhua ;
Qiao, L. ;
Zhong, Cuiping ;
Xue, Tao ;
Wang, Menghang .
HYBRIDOMA, 2008, 27 (03) :187-190
[3]  
HONG JL, DIS ESOPHAGUS, DOI DOI 10.1111/J.1442-2050.2007.00803.X
[4]   Zinc ribbon domain-containing 1 (ZNRD1) mediates multidrug resistance of leukemia cells through regulation of P-glycoprotein and Bcl-2 [J].
Hong, L ;
Piao, Y ;
Han, Y ;
Wang, J ;
Zhang, XY ;
Du, YL ;
Cao, SS ;
Qiao, TD ;
Chen, Z ;
Fan, DM .
MOLECULAR CANCER THERAPEUTICS, 2005, 4 (12) :1936-1942
[5]  
Hong L, 2005, CANCER BIOL THER, V4, P60
[6]   Reversal of multidrug resistance of gastric cancer cells by down-regulation of ZNRD1 with ZNRD1 siRNA [J].
Hong, L ;
Ning, X ;
Shi, Y ;
Shen, H ;
Zhang, Y ;
Lan, M ;
Liang, S ;
Wang, J ;
Fan, D .
BRITISH JOURNAL OF BIOMEDICAL SCIENCE, 2004, 61 (04) :206-210
[7]   Suppression of the cell proliferation in stomach cancer cells by the ZNRD1 gene [J].
Hong, L ;
Zhang, YM ;
Liu, N ;
Liu, CJ ;
Zhi, M ;
Pan, YL ;
Lan, M ;
Sun, L ;
Fan, DM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 321 (03) :611-616
[8]   ZNRD1 mediates resistance of gastric cancer cells to methotrexate by regulation of IMPDH2 and Bcl-2 [J].
Hong, Liu ;
Qiao, Taidong ;
Han, Yu ;
Han, Shuang ;
Zhang, Xiaoyin ;
Lin, Tao ;
Gao, Juan ;
Zhao, Pengtao ;
Chen, Zhen ;
Fan, Daiming .
BIOCHEMISTRY AND CELL BIOLOGY, 2006, 84 (02) :199-206
[9]   A nonconserved surface of the TFIIB zinc ribbon domain plays a direct role in RNA polymerase II recruitment [J].
Tubon, TC ;
Tansey, WP ;
Herr, W .
MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (07) :2863-2874
[10]   Cryoembedding and sectioning of cochleas for immunocytochemistry and in situ hybridization [J].
Whitlon, DS ;
Szakaly, R ;
Greiner, MA .
BRAIN RESEARCH PROTOCOLS, 2001, 6 (03) :159-166
12