Detoxification roles of tributyltin-binding protein type 2 in Japanese medaka (Oryzias latipes) exposed to tributyltin

被引：14

作者：

Qiu, Xuchun ^{[1
,2
]}

Takamura, Takumi ^{[2
,3
]}

Enoki, Shintaro ^{[2
]}

Kato-Unoki, Yoko ^{[4
]}

Takai, Yuki ^{[2
]}

Nagano, Yosuke ^{[2
]}

Kinoshita, Masato ^{[5
]}

Kitano, Takeshi ^{[6
]}

Shimasaki, Yohei ^{[2
]}

Oshima, Yuji ^{[2
,7
]}

机构：

[1] Jiangsu Univ, Inst Environm Hlth & Ecol Secur, Zhenjiang 212013, Jiangsu, Peoples R China

[2] Kyushu Univ, Lab Marine Environm Sci, Fac Agr, Fukuoka 8190395, Japan

[3] Chem Evaluat & Res Inst, 3-2-7 Miyanojin, Kurume, Fukuoka 8390801, Japan

[4] Kyushu Univ, Ctr Adv Instrumental & Educ Supports, Fac Agr, Fukuoka 8190395, Japan

[5] Kyoto Univ, Grad Sch Agr, Dept Appl Biosci, Kyoto 6068502, Japan

[6] Kumamoto Univ, Grad Sch Sci & Technol, Dept Biol Sci, Kumamoto 8608555, Japan

[7] Kanazawa Univ, Inst Nat & Environm Technol, Kakuma Machi, Kanazawa, Ishikawa 9201192, Japan

来源：

MARINE POLLUTION BULLETIN | 2020年 / 159卷

基金：

日本学术振兴会;

关键词：

Detoxification; Genome editing; Medaka; Tributyltin-binding protein; Tributyltin; ORGANOTIN COMPOUNDS; GENE-EXPRESSION; FLOUNDER; TOXICITIES; ACCUMULATION; GLYCOPROTEIN; PURIFICATION; MIXTURES; BLOOD;

D O I：

10.1016/j.marpolbul.2020.111445

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Tributyltin-binding protein type 2 (TBT-bp2), a homolog of alpha 1-acid glycoprotein, may contribute to both accumulation and detoxification of TBT in fish. In this study, we conducted acute TBT exposure tests using both wide-type (WT) and TBT-bp2(-/-) (KO) strains of medaka and compared their responses in survival time and accumulation of TBT. Deficiency of TBT-bp2 significantly accelerated the time to death of medaka and decreased the LC50 of TBT, indicating that the KO-strain is more sensitive to TBT. No significant difference in the intrinsic TBT concentration in surviving fish was observed between the two strains. However, the intrinsic TBT concentration in dead KO-strain was significantly lower than that in WT-strain. These findings provide direct evidence, supporting the hypothesis that TBT-bp2 plays a critical role in the detoxification of TBT in fish.

引用

页数：4

共 31 条

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