Detection of infections with hepatitis B virus, hepatitis C virus, and human immunodeficiency virus by analyses of dried blood spots - performance characteristics of the ARCHITECT system and two commercial assays for nucleic acid amplification

被引：62

作者：

Ross, R. Stefan ^{[1
]}

Stambouli, Oumaima ^{[1
]}

Gruener, Nico ^{[1
]}

Marcus, Ulrich ^{[2
]}

Cai, Wei ^{[2
]}

Zhang, Weidong ^{[2
]}

Zimmermann, Ruth ^{[2
]}

Roggendorf, Michael ^{[1
]}

机构：

[1] Univ Duisburg Essen, Inst Virol, Natl Reference Ctr Hepatitis C, Univ Hosp Essen, Essen, Germany

[2] Robert Koch Inst, Dept Infect Dis Epidemiol, Berlin, Germany

来源：

VIROLOGY JOURNAL | 2013年 / 10卷

关键词：

Hepatitis B virus infection; Hepatitis C virus infection; Human immunodeficiency virus infection; Dried blood spots; Intravenous drug users; Serological testing; Molecular testing; Real-time PCR; Transcription-mediated amplification; INJECTING DRUG-USERS; REAL-TIME PCR; ANTIBODY DETECTION; RNA; DNA; PREVALENCE; DIAGNOSIS; MARKERS; SAMPLES; QUANTIFICATION;

D O I：

10.1186/1743-422X-10-72

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Nowadays, dried blood spots (DBS) are primarily used to obtain diagnostic access to risk collectives such as intravenous drug users, who are prone to infections with hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). Before DBS analyses can be used in this diagnostic context, however, a comprehensive evaluation of its performance characteristics must be conducted. To the best of our knowledge, the current study presents for the first time such essential data for the Abbott ARCHITECT system, which is currently the worldwide leading platform in this field of infection diagnostics. Methods: The investigation comprised 1,762 paired serum/DBS samples and a total of 3,524 determinations with the Abbott ARCHITECT HBsAg, anti-HBc, anti-HBs, anti-HCV and HIV-1-p24-antigen/anti-HIV 1/2 assays as well as with the artus HBV LC PCR and VERSANT HCV RNA qualitative (TMA) tests. Results: In the context of DBS testing, a specificity of 100% was recorded for the seven serological and molecular biological assays. The analytical sensitivity of HBsAg, anti-HBc, anti-HBs, anti-HCV, HIV-1-p24-antigen/anti-HIV 1/2, HBV DNA, and HCV RNA detections in DBS eluates was 98.6%, 97.1%, 97.5%, 97.8%, 100%, 93%, and 100%, respectively. Discussion/conclusions: The results obtained indicate that it is today possible to reliably detect HBsAg, anti-HBc, anti-HBs, anti-HCV and HIV-1-p24 antigen/anti-HIV 1/2 with state-of-the-art analytical systems such as the Abbott ARCHITECT in DBS eluates even when a comparatively high elution volume of 1,000 mu l is used. They also provide evidence for the inherent analytical limits of DBS testing, which primarily concern the anti-HBc/anti-HBs system for individuals with HIV infections and nucleic acid tests with relatively low analytical sensitivity.

引用

页数：8

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[1] Global estimates of prevalence of HCV infection among injecting drug users [J].