Preparation and evaluation of open tubular electrochromatography column with derivated polysiloxane as stationary phase

A simple and valid procedure for the preparation of open tubular electrochromatography column with derivated polysiloxane as stationary phase was established. When using polysiloxane derivated with butyl methacrylate and vinylsulfonic acid as stationary phase, the column has a strong ability for separation of the series of benzene. The separation efficiency of tuluene was 1.52 X 10(5) theoretical plates/meter and the retention factors of the series of benzene were larger than 2.40. After two months, there was no obvious difference in the separation ability of the column. In this method, the polymer,was directly bonded in 50 mum i.d. fused-silica capillary without pre-etching. When using polysiloxane derivated with allyl-permethyl-beta-cyclodextrin and vinylsulfonic acid as chiral stationary phase, naproxen could be enantiomeric separated with resolution of 0.81. In this methods the 50 mum i. d. fuse-silica capillary was primarily treated with Sol-Gel technology to increase the area of inner wall and the density of Si-OH.