Influence of phytic acid and its metal complexes on the activity of pectin degrading polygalacturonase

被引：6

作者：

Asghar, Uzma ^{[1
]}

Rehman, Haneef Ur ^{[2
]}

Ul Qader, Shah Ali ^{[2
]}

Maqsood, Zahida Tasneem ^{[1
]}

机构：

[1] Univ Karachi, Dept Chem, Karachi 75270, Pakistan

[2] Univ Karachi, KIBGE, Karachi 75270, Pakistan

来源：

CARBOHYDRATE POLYMERS | 2013年 / 95卷 / 01期

关键词：

Polygalacturonase; Bacillus licheniformis; Phytic acid; Metallic cations; Phytate-metal cations complexes; ENZYMES;

D O I：

10.1016/j.carbpol.2013.02.065

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Polygalacturonase is one of the important requirements of different microorganism to cause pathogenicity and spoilage of fruits and vegetables that involved in degradation of pectin during plant tissue infections. In current study, 20 mM phytic acid inhibited 70% activity of polygalacturonase. The effect of different concentration of metal ions such as Cu+2, Al+3 and V+4 were studied separately and it was found that the 20 mM of these metal ions inhibited 37.2%, 79%, and 53% activity of polygalacturonase, respectively. Finally, the complexes of phytic acid and these metals ions were prepared and 1:1 ratio of phytic acid and metal ions complexes showed maximum inhibitory activity of enzyme as compared to complexes having 1:2 and 1:3 ratio except phytate-copper complexes which showed no inhibitory effect on the activity of polygalacturonase. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：167 / 170

页数：4

共 15 条

[11] USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR [J].