Pollen morphology in delimiting subgenera and species of the genus Cybianthus s.l. (Myrsinoideae-Primulaceae)

Pollen grains of species of the subgenera of Cybianthus s.l. were analyzed in order to determine if pollen morphology is consistent with the currently accepted infrageneric classification, and if it can provide characters useful for distinguishing species or groups of species. Acetolyzed pollen grains were analyzed, measured, described and photomicrographed, while non-acetolyzed pollen grains were analyzed using scanning electron microscopy. The pollen grains of all species investigated were isopolar monads, small- to medium-sized, subprolate or prolate, 3-colporate, and rugulate or rugulate-perforate. The different analytical methods (statistical analyses, exploratory analysis of the data and cluster analysis) used demonstrated that pollen morphology is informative in delimiting this genus. However, the pollen characters are generally quite uniform among the subgenres of Cybianthus s.l. Thus, the use of the pollen attributes in the separation of the subordinate species to different subgenera is not effective. Pollen morphology did not give support to current subgeneric systematics, but it did support the genus Cybianthus s.l.